Birtles Andrew, McCarthy Noel, Sheppard Carmen L, Rutter Harry, Guiver Malcolm, Haworth Elizabeth, George Robert C
Health Protection Agency, Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK.
J Clin Microbiol. 2005 Apr;43(4):2004-8. doi: 10.1128/JCM.43.4.2004-2008.2005.
Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
对两例明显相关的肺炎球菌性脑膜炎致死病例进行了调查。在一例培养阴性的病例中,对血液和脑脊液进行的肺炎溶素聚合酶链反应(PCR)呈阳性。第二例病例血培养分离出肺炎链球菌。对从病例1(标本)和病例2(分离株)提取的DNA进行多位点序列分型,结果显示序列类型相同(ST53),证实了两者之间的关联。