使用多重 PCR 结合脑脊液对非洲脑膜炎带的肺炎球菌性脑膜炎病例进行血清分型。
Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid.
机构信息
Agence de Médecine Préventive, at the Institut Pasteur, 25-28 Rue du Dr. Roux, 75724 Paris Cedex 15, France.
出版信息
J Clin Microbiol. 2010 Feb;48(2):612-4. doi: 10.1128/JCM.01402-09. Epub 2009 Dec 9.
Abstract
We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.
摘要
我们重新设计了一种用于直接从脑脊液 (CSF) 中对肺炎球菌性脑膜炎进行血清分型的多重 PCR 算法。与分离株的既定方法相比，基于 CSF 的 PCR 具有至少 80%的灵敏度和 100%的特异性。在区域性脑膜炎监测中，基于 CSF 的 PCR 将血清型信息的检出率从 40%（分离物检测）提高到 90%。
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