Opatrný K, Spustová V, Rusavý Z, Opatrný K, Haschová M, Eiselt J, Válek A
Interní oddĕlení Strahov, Praha.
Cas Lek Cesk. 1992 Mar 13;131(5):142-5.
The objective of the investigation was to assess whether therapeutic membrane plasmapheresis accelerates protein synthesis. To this end pseudouridine (PSI), a modified nucleoside was investigated which provides information on the tRNA turnover and thus indirectly also on protein synthesis. The authors made 10 plasmapheresis on a A 2008 PF monitor with Plasmaflux P2 filters which they use to exchange 1 plasma volume of the patients. Laboratory indicators were investigated one day before plasmapheresis, on the day of plasmapheresis and during its course, and on the 1st, 2nd and possibly 3rd day after plasmapheresis. They revealed that the clearance of the plasma filter for PSI (0.41 +/- 0.04 ml/s, arithmetical mean +/- SEM) did not differ significantly from the filtration rate (0.49 +/- 0.01 ml/s, p = 0.15). As compared with the initial examination (0.49 +/- 0.06 ml/s) on the first day after plasmapheresis as a result of reduced glomerular filtration rate the renal clearance of PSI was reduced (0.33 +/- 0.05, p less than 0.01). PSI serum concentrations were therefore expressed as the serum PSI/serum creatinine ratio. This ratio was, as compared with the initial examination (80.4 +/- 4.8 nmol/mumol), raised midway during the procedure (100.8 +/- 8.6, p much less than 0.05) and after its termination (132.3 +/- 6.1, p much less than 0.01). The increase was not due to disintegration of cells or dietary factors. The rise of the serum PSI/serum creatinine ratio was due to a more rapid tRNA turnover and thus provided evidence that therapeutic membrane plasmapheresis accelerates protein synthesis.
该研究的目的是评估治疗性膜血浆置换是否能加速蛋白质合成。为此，研究了一种修饰核苷假尿苷（PSI），它可提供有关tRNA周转的信息，从而也间接提供有关蛋白质合成的信息。作者使用Plasmaflux P2滤器在A 2008 PF监测仪上进行了10次血浆置换，用于置换患者1个血浆量。在血浆置换前一天、血浆置换当天及其过程中以及血浆置换后第1天、第2天甚至可能第3天对实验室指标进行了研究。他们发现，血浆滤器对PSI的清除率（0.41±0.04 ml/s，算术平均值±标准误）与过滤速率（0.49±0.01 ml/s，p = 0.15）无显著差异。与血浆置换后第一天的初始检查（0.49±0.06 ml/s）相比，由于肾小球滤过率降低，PSI的肾清除率降低（0.33±0.05，p＜0.01）。因此，PSI血清浓度表示为血清PSI/血清肌酐比值。与初始检查（80.4±4.8 nmol/μmol）相比，该比值在操作过程中（100.8±8.6，p远＜0.05）和操作结束后（132.3±6.1，p远＜0.01）有所升高。这种升高不是由于细胞分解或饮食因素。血清PSI/血清肌酐比值的升高是由于tRNA周转加快，从而提供了治疗性膜血浆置换加速蛋白质合成的证据。
