Liu Wei, Chen Jian-Hai, Ren Fei, Guo Yun-Bo, Yan Xi-Qing, Liu Yan-Dong
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Apr;25(4):413-5.
To establish a method by high-performance liquid chromatography (HPLC) for determining the concentration of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma.
Chromatography was performed on a LiChroCART C18 (250 mm x 4 mm, 5 microm) column with the mobile phase consisting of acetonitrile-NaAC (15:85), the flow rate of 1.0 ml/min, and the detection wavelength of 365 nm. Sample extraction was carried out with ethylacetate.
The linear range of mouse plasma mitomycin C concentration was 0.04-1.00 microg/ml, and the linear equation of Y=16 388X-17.17 (r=0.999 8) was derived.
This method is very easy to operate and suits the need of perclinical pharmacokinetic studies of mitomycin-magnetic nanoparticles and yields accurate and precise results.
建立一种高效液相色谱法（HPLC）测定小鼠血浆中磁性丝裂霉素C-聚氰基丙烯酸正丁酯纳米粒的浓度。
采用LiChroCART C18柱（250 mm×4 mm，5μm）进行色谱分析，流动相为乙腈-醋酸钠（15:85），流速为1.0 ml/min，检测波长为365 nm。用乙酸乙酯进行样品提取。
小鼠血浆丝裂霉素C浓度的线性范围为0.04 - 1.00μg/ml，得出线性方程Y = 16388X - 17.17（r = 0.9998）。
该方法操作简便，适用于丝裂霉素磁性纳米粒临床前药代动力学研究的需要，结果准确、精密。
