Taguchi Kazuhiro, Ogawa Rei, Migita Makoto, Hanawa Hideki, Ito Hiromoto, Orimo Hideo
Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan.
Biochem Biophys Res Commun. 2005 May 27;331(1):31-6. doi: 10.1016/j.bbrc.2005.03.119.
We investigated the role of bone marrow cells in bone fracture repair using green fluorescent protein (GFP) chimeric model mice. First, the chimeric model mice were created: bone marrow cells from GFP-transgenic C57BL/6 mice were injected into the tail veins of recipient wild-type C57BL/6 mice that had been irradiated with a lethal dose of 10Gy from a cesium source. Next, bone fracture models were created from these mice: closed transverse fractures of the left femur were produced using a specially designed device. One, three, and five weeks later, fracture lesions were extirpated for histological and immunohistochemical analyses. In the specimens collected 3 and 5 weeks after operation, we confirmed calluses showing intramembranous ossification peripheral to the fracture site. The calluses consisted of GFP- and osteocalcin-positive cells at the same site, although the femur consisted of only osteocalcin-positive cells. We suggest that bone marrow cells migrated outside of the bone marrow and differentiated into osteoblasts to make up the calluses.
我们使用绿色荧光蛋白（GFP）嵌合模型小鼠研究了骨髓细胞在骨折修复中的作用。首先，创建嵌合模型小鼠：将来自GFP转基因C57BL/6小鼠的骨髓细胞注射到接受了来自铯源的10Gy致死剂量照射的野生型C57BL/6受体小鼠的尾静脉中。接下来，从这些小鼠创建骨折模型：使用专门设计的装置造成左股骨闭合性横行骨折。1周、3周和5周后，取出骨折损伤部位进行组织学和免疫组织化学分析。在术后3周和5周收集的标本中，我们确认在骨折部位周围有显示膜内成骨的骨痂。尽管股骨仅由骨钙素阳性细胞组成，但骨痂在同一部位由GFP阳性和骨钙素阳性细胞组成。我们认为骨髓细胞迁移到骨髓外并分化为成骨细胞以形成骨痂。
