Patel Pratik, Shanthraj Ravi, Bhagyalakshmi A, Garg Nekta, Vallakati Anisha
Department of Orthodontics, JSS Dental College and Hospital, JSS University, Mysore, Karnataka, India.
J Orthod Sci. 2016 Apr-Jun;5(2):52-6. doi: 10.4103/2278-0203.179407.
To compare the levels of pentraxin 3 (PTX-3) in gingival crevicular fluid (GCF) in patients undergoing orthodontic canine retraction with active tieback and nickel titanium (NiTi) coil spring.
Fifteen patients of the age group 15-25 years with first premolar extraction undergoing canine retraction were selected. One month after placement of 0.019" × 0.025" stainless steel wire, canine retraction was started with active tieback (150 g force) on upper right quadrant and NiTi coil spring (150 g force) on upper left quadrant. GCF samples were collected 1 h before commencement of canine retraction and thereafter at intervals of 1 h, 1 day, 1 week, and 2 weeks after application of force. The collected GCF was eluted from the microcapillary pipette in 100 μl phosphate-buffered saline (pH 5-7.2). The samples were analyzed for PTX-3 levels by the ELISA technique.
The mean levels of PTX-3 at 1 h before canine retraction (baseline) was 1.30 ± 0.22 ng/ml and at 1 h 1.66 ± 0.33 ng/ml, 1 day 2.65 ± 0.09 ng/ml, 1 week 1.96 ± 0.15 ng/ml, and 2 weeks 1.37 ± 0.18 ng/ml in active tieback group. The mean levels of PTX-3 at 1 h before canine retraction was 1.32 ± 0.30 ng/ml, and at 1 h 1.71 ± 0.39 ng/ml, 1 day 2.78 ± 0.12 ng/ml, 1 week 2.52 ± 0.18 ng/ml, and 2 weeks 2.12 ± 0.17 ng/ml in NiTi coil spring group. A significant difference of P < 0.001 was found in PTX-3 levels in GCF during canine retraction between active tieback and NiTi coil spring at 1 day, 1 week, and 2 weeks.
The results showed that PTX-3 levels increased from 1 h after application of orthodontic force and reached peak at 1 day, followed by a gradual decrease at 1 week and 2 weeks in both active tie back and NiTi coil spring groups.
比较使用活动结扎丝和镍钛（NiTi）螺旋弹簧进行正畸犬牙后移的患者龈沟液（GCF）中五聚体3（PTX-3）的水平。
选取15例年龄在15至25岁之间、正在进行第一前磨牙拔除并犬牙后移的患者。在放置0.019"×0.025"不锈钢丝1个月后，开始进行犬牙后移，右上象限使用活动结扎丝（150克力），左上象限使用NiTi螺旋弹簧（150克力）。在犬牙后移开始前1小时以及施力后1小时、1天、1周和2周时收集GCF样本。将收集到的GCF从微量毛细管移液器中洗脱到100μl磷酸盐缓冲盐水（pH 5 - 7.2）中。通过ELISA技术分析样本中的PTX-3水平。
在活动结扎丝组中，犬牙后移前1小时（基线）PTX-3的平均水平为1.30±0.22 ng/ml，1小时时为1.66±0.33 ng/ml，1天时为2.65±0.09 ng/ml，1周时为1.96±0.15 ng/ml，2周时为1.37±0.18 ng/ml。在NiTi螺旋弹簧组中，犬牙后移前1小时PTX-3的平均水平为1.32±0.30 ng/ml，1小时时为1.71±0.39 ng/ml，1天时为2.78±0.12 ng/ml，1周时为2.52±0.18 ng/ml，2周时为2.12±0.17 ng/ml。在犬牙后移期间，活动结扎丝组和NiTi螺旋弹簧组在1天、1周和2周时GCF中PTX-3水平的差异具有统计学意义（P < 0.001）。
结果表明，在活动结扎丝组和NiTi螺旋弹簧组中，正畸力施加后1小时PTX-3水平开始升高，1天时达到峰值，随后在1周和2周时逐渐下降。
