Kalicharran K K, Springthorpe V S, Sattar S A
Department of Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario.
Can J Vet Res. 1992 Jan;56(1):28-33.
Human adenovirus type 5 containing the rabies virus glycoprotein gene (rHAd-RG1) has potential for the oral vaccination of animals. The stability of this recombinant was tested indoors and outdoors by measuring the loss in virus infectivity. Under indoor conditions the stability of the recombinant virus was studied in an egg yolk-containing commercial stabilizer and a simple buffered salt solution (EBSS; Earle's balanced salt solution) at 4 degrees C and room temperature (24-25 degrees C). Over 16 days, there was a more rapid loss in virus titer at room temperature than at 4 degrees C in both suspending media; however, these differences were slight and may be significant when the overall stability of the vaccine is considered. When the virus was mixed with either 10% (w/v) fox or skunk feces or EBSS, placed on stainless steel disks and the disks kept under ambient conditions (air temperature 24-25 degrees C; relative humidity 45-50%), there was a more rapid decline in virus titer in the fecal suspensions (3% remained after 72 h) than in EBSS (26% remained after 72 h). When bait-coated blister packs of the vaccine were placed in an outdoor location in the fall (October) season, there was a larger drop in the virus titer for vaccines placed in the sun (54% over 32 days) than for those in the shade (40% over 32 days). Incorporating proteinaceous stabilizers in the vaccine samples for outdoor study showed virus stability was not enhanced in their presence.(ABSTRACT TRUNCATED AT 250 WORDS)
携带狂犬病病毒糖蛋白基因的5型人腺病毒（rHAd-RG1）具有用于动物口服疫苗接种的潜力。通过测量病毒感染力的损失，在室内和室外对该重组体的稳定性进行了测试。在室内条件下，在含蛋黄的商业稳定剂和简单的缓冲盐溶液（EBSS；Earle平衡盐溶液）中，于4℃和室温（24 - 25℃）下研究重组病毒的稳定性。在16天内，在两种悬浮介质中，室温下病毒滴度的下降都比4℃时更快；然而，这些差异很小，在考虑疫苗的整体稳定性时可能具有显著性。当病毒与10%（w/v）的狐狸或臭鼬粪便或EBSS混合，置于不锈钢盘上并将盘置于环境条件下（气温24 - 25℃；相对湿度45 - 50%）时，粪便悬浮液中病毒滴度的下降（72小时后3%残留）比在EBSS中更快（72小时后26%残留）。当秋季（10月）将包被诱饵的疫苗泡罩包装放置在室外时，置于阳光下的疫苗病毒滴度下降幅度（32天内下降54%）大于置于阴凉处的疫苗（32天内下降40%）。在用于室外研究的疫苗样品中加入蛋白质稳定剂后发现，其存在并未增强病毒的稳定性。（摘要截短于250字）
