Blancou J, Kieny M P, Lathe R, Lecocq J P, Pastoret P P, Soulebot J P, Desmettre P
Nature. 1986;322(6077):373-5. doi: 10.1038/322373a0.
Rabies, a viral disease affecting all warm-blooded animals, is prevalent in most parts of the world, where it propagates amongst wild animals, particularly the fox and dog. The public health and economic consequences of infection in man and livestock are well known. Attempts to control the disease by vaccinating wild carnivores with inactivated or attenuated rabies virus remain controversial, and we have instead evaluated here the potential of a recombinant vaccinia virus to protect foxes against the disease. We have found that the administration of vaccinia virus (VV) or a recombinant harbouring the rabies surface antigen gene (VVTGgRAB) is innocuous to foxes. The recombinant virus can elicit the production of titers of rabies-neutralizing antibodies equal or superior to those obtained with conventional vaccine, and 10(8) plaque-forming units (PFU) of VVTGgRAB administered subcutaneously, intradermally or orally confers complete protection to severe challenge infection with street rabies virus.
狂犬病是一种影响所有温血动物的病毒性疾病，在世界大部分地区都很普遍，它在野生动物中传播，尤其是狐狸和狗。人类和牲畜感染狂犬病对公共卫生和经济造成的后果是众所周知的。通过给野生食肉动物接种灭活或减毒狂犬病病毒来控制该疾病的尝试仍然存在争议，因此我们在此评估了重组痘苗病毒保护狐狸免受该病侵害的潜力。我们发现，接种痘苗病毒（VV）或携带狂犬病表面抗原基因的重组病毒（VVTGgRAB）对狐狸是无害的。这种重组病毒能够引发狂犬病中和抗体的产生，其滴度与传统疫苗相当或更高，皮下、皮内或口服给予10⁸ 蚀斑形成单位（PFU）的VVTGgRAB可使狐狸完全抵御街狂犬病毒的严重攻击感染。
