Blasi E, Pitzurra L, Puliti M, Bartoli A, Bistoni F
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Cell Immunol. 1992 Jun;142(1):137-44. doi: 10.1016/0008-8749(92)90275-t.
We have demonstrated that Candida albicans in its hyphal form (H-Candida) acts as a stimulating agent in the cloned macrophage population ANA-1. Both tumor necrosis factor (TNF) mRNA levels and secreted biological activity augment in ANA-1 macrophages exposed to H-Candida. Such effects are observed at an effector-to-target cell ratio of 1:1 and occur after 1 and 3 hr of coincubation, respectively. The phenomenon is independent of the metabolic status of the fungus, since viable as well as heat-killed H-Candida are comparable in inducing TNF mRNA levels. The extent and kinetics of H-Candida-mediated effects are similar to those observed following exposure of ANA-1 macrophages to lipopolysaccharide (LPS). This implies that C. albicans in its hyphal form is a potent macrophage modulator; whether it acts through the same mechanism(s) as LPS remains to be elucidated.
我们已经证明，白色念珠菌的菌丝形态（H-念珠菌）在克隆的巨噬细胞群体ANA-1中充当刺激剂。在暴露于H-念珠菌的ANA-1巨噬细胞中，肿瘤坏死因子（TNF）的mRNA水平和分泌的生物活性均会增强。在效应细胞与靶细胞比例为1:1时观察到这种效应，并且分别在共孵育1小时和3小时后出现。该现象与真菌的代谢状态无关，因为活的和热灭活的H-念珠菌在诱导TNF mRNA水平方面具有可比性。H-念珠菌介导的效应的程度和动力学与将ANA-1巨噬细胞暴露于脂多糖（LPS）后观察到的相似。这意味着菌丝形态的白色念珠菌是一种有效的巨噬细胞调节剂；它是否通过与LPS相同的机制起作用还有待阐明。
