Caballero-Mellado Jesús, Martínez-Aguilar Lourdes, Paredes-Valdez Guadalupe, Santos Paulina Estrada-de Los
Programa de Ecología Molecular y Microbiana, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Apdo. Postal 565-A, Cuernavaca, Morelos, México.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1165-1172. doi: 10.1099/ijs.0.02951-0.
It was shown recently that the genus Burkholderia is rich in N2-fixing bacteria that are associated with plants. A group of these diazotrophic isolates with identical or very similar 16S rDNA restriction patterns [designated amplified rDNA restriction analysis (ARDRA) genotypes 13, 14 and 15] was selected and a polyphasic taxonomic study was performed, which included new isolates that were recovered from rhizospheres, rhizoplanes or internal tissues of maize, sugarcane and coffee plants. Morphological, physiological and biochemical features, as well as multi-locus enzyme electrophoresis profiles and whole-cell protein patterns, of 20 strains were analysed. In addition, analysis of cellular fatty acid profiles, 16S rDNA sequence analysis and DNA-DNA reassociation experiments were performed with representative strains. The taxonomic data indicated that the strains analysed belong to a novel diazotrophic Burkholderia species, for which the name Burkholderia unamae sp. nov. is proposed. Strain MTl-641T (=ATCC BAA-744T=CIP 107921T), isolated from the rhizosphere of maize, was designated as the type strain. B. unamae was found as an endophyte of plants grown in regions with climates ranging from semi-hot subhumid to hot humid, but not from plants grown in regions with semi-hot or hot dry climates. Moreover, B. unamae was isolated from rhizospheres and plants growing in soils with pH values in the range 4.5-7.1, but not from soils with pH values higher than 7.5.
最近的研究表明，伯克霍尔德氏菌属富含与植物相关的固氮细菌。从这些具有相同或非常相似的16S rDNA限制性图谱（指定为扩增rDNA限制性分析（ARDRA）基因型13、14和15）的固氮分离物中选取一组，并进行了多相分类学研究，其中包括从玉米、甘蔗和咖啡植物的根际、根表或内部组织中分离得到的新分离物。分析了20株菌株的形态、生理和生化特征，以及多位点酶电泳图谱和全细胞蛋白质模式。此外，还对代表性菌株进行了细胞脂肪酸谱分析、16S rDNA序列分析和DNA-DNA重联实验。分类学数据表明，所分析的菌株属于一个新的固氮伯克霍尔德氏菌物种，为此提出了伯克霍尔德氏菌新种（Burkholderia unamae sp. nov.）的名称。从玉米根际分离得到的菌株MTl-641T（=ATCC BAA-744T=CIP 107921T）被指定为模式菌株。在气候从半热亚湿润到热湿润的地区生长的植物中发现了Unamae伯克霍尔德氏菌作为内生菌，但在半热或热干燥气候地区生长的植物中未发现。此外，Unamae伯克霍尔德氏菌是从pH值在4.5-7.1范围内的土壤中的根际和植物中分离得到的，但在pH值高于7.5的土壤中未分离到。
