Reis V M, Santos P Estrada-de Los, Tenorio-Salgado S, Vogel J, Stoffels M, Guyon S, Mavingui P, Baldani V L D, Schmid M, Baldani J I, Balandreau J, Hartmann A, Caballero-Mellado J
Centro Nacional de Pesquisa de Agrobiologia (EMBRAPA-Agrobiologia), km 47, Seropédica, 23851-970, CP 74505, Rio de Janeiro, Brazil.
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, México.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2155-2162. doi: 10.1099/ijs.0.02879-0.
In an ecological survey of nitrogen-fixing bacteria isolated from the rhizosphere and as endophytes of sugarcane, maize and teosinte plants in Brazil, Mexico and South Africa, a new phylogenetically homogeneous group of N(2)-fixing bacteria was identified within the genus Burkholderia. This polyphasic taxonomic study included microscopic and colony morphology, API 20NE tests and growth on different culture media at different pH and temperatures, as well as carbon source assimilation tests and whole-cell protein pattern analysis. Analysis of 16S rRNA gene sequences showed 99.2-99.9 % similarity within the novel species and 97.2 % similarity to the closest related species, Burkholderia sacchari. The novel species was composed of four distinct amplified 16S rDNA restriction analysis groups. The DNA-DNA reassociation values within the novel species were greater than 70 % and less than 42 % for the closest related species, B. sacchari. Based on these results and on many phenotypic characteristics, a novel N(2)-fixing species is proposed for the genus Burkholderia, Burkholderia tropica sp. nov., with the type strain Ppe8(T) (=ATCC BAA-831(T)=LMG 22274(T)=DSM 15359(T)). B. tropica was isolated from plants grown in geographical regions with climates ranging from temperate subhumid to hot humid.
在一项针对从巴西、墨西哥和南非的甘蔗、玉米和大刍草植物根际及内生环境中分离出的固氮细菌的生态学调查中，在伯克霍尔德菌属内鉴定出了一个新的系统发育均一的固氮细菌群体。这项多相分类研究包括微观和菌落形态、API 20NE测试、在不同pH值和温度下在不同培养基上的生长情况，以及碳源同化测试和全细胞蛋白质模式分析。16S rRNA基因序列分析显示，新物种内的相似性为99.2 - 99.9%，与最密切相关的物种伯克霍尔德菌属甘蔗伯克霍尔德菌的相似性为97.2%。新物种由四个不同的16S rDNA扩增限制性分析组组成。新物种内的DNA - DNA重结合值大于70%，与最密切相关的物种甘蔗伯克霍尔德菌的重结合值小于42%。基于这些结果以及许多表型特征，提议为伯克霍尔德菌属设立一个新的固氮物种，热带伯克霍尔德菌（Burkholderia tropica sp. nov.），模式菌株为Ppe8(T)（=ATCC BAA - 831(T)=LMG 22274(T)=DSM 15359(T)）。热带伯克霍尔德菌是从生长在气候范围从温带亚湿润到炎热湿润的地理区域的植物中分离出来的。
