Shen Fo-Ting, Kämpfer Peter, Young Chiu-Chung, Lai Wei-An, Arun A B
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1301-1304. doi: 10.1099/ijs.0.63514-0.
A bacterial strain (CC-TWGS1-8(T)) isolated from a tar-contaminated soil in Taiwan was studied in a detailed taxonomic study. The cells were Gram-negative, rod-shaped and non-spore-forming. Phylogenetic analyses using the 16S rRNA gene sequence of the strain clearly revealed an affiliation to the genus Chryseobacterium, the highest sequence similarities being to the type strain of Chryseobacterium indologenes (96.8 %), to Chryseobacterium gleum (96.8 %) and to Chryseobacterium joostei (96.4 %). The 16S rRNA gene sequence similarities to all other Chryseobacterium species were below 96 %. The major whole-cell fatty acids were 15 : 0 iso (35.4 %) and 17 : 0 iso 3OH (22.5 %). DNA-DNA hybridization values and the biochemical and chemotaxonomic properties demonstrate that strain CC-TWGS1-8(T) represents a novel species, for which the name Chryseobacterium taichungense sp. nov. is proposed. The type strain is CC-TWGS1-8(T) (= CCUG 50001(T) = CIP 108519(T)).
对从台湾受焦油污染土壤中分离出的一株细菌（CC-TWGS1-8(T)）进行了详细的分类学研究。该菌细胞呈革兰氏阴性，杆状，不产芽孢。利用该菌株的16S rRNA基因序列进行的系统发育分析清楚地表明它属于金黄杆菌属，与产吲哚金黄杆菌模式菌株的序列相似性最高（96.8%），与格氏金黄杆菌（96.8%）和乔氏金黄杆菌（96.4%）的相似性也较高。与所有其他金黄杆菌属物种的16S rRNA基因序列相似性均低于96%。主要的全细胞脂肪酸为15 : 0异（35.4%）和17 : 0异3OH（22.5%）。DNA-DNA杂交值以及生化和化学分类学特性表明菌株CC-TWGS1-8(T)代表一个新物种，为此提出新名称台中金黄杆菌（Chryseobacterium taichungense sp. nov.）。模式菌株为CC-TWGS1-8(T)（= CCUG 50001(T) = CIP 108519(T)）。
