Kämpfer Peter, McInroy John A, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany,
Antonie Van Leeuwenhoek. 2015 Feb;107(2):533-8. doi: 10.1007/s10482-014-0349-3. Epub 2014 Dec 17.
A slightly yellow pigmented strain (JM-534(T)) isolated from the rhizoplane of a field-grown Zea mays plant was investigated using a polyphasic approach for its taxonomic allocation. Cells of the isolate were observed to be rod-shaped and to stain Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to Chryseobacterium lactis (98.9 %), Chryseobacterium joostei and Chryseobacterium indologenes (both 98.7 %), and Chryseobacterium viscerum (98.6 %). Sequence similarities to all other Chryseobacterium species were 98.5 % or below. The fatty acid analysis of the strain resulted in a Chryseobacterium typical pattern consisting mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA-DNA hybridizations with the type strains of C. lactis, C. joostei, C. viscerum and C. indologenes resulted in values below 70 %. Genomic fingerprinting showed that the isolate was very different to the type strains of these species. Differentiating biochemical and chemotaxonomic properties showed that the isolate JM-534(T) represents a novel species, for which the name Chryseobacterium rhizoplanae sp. nov. (type strain JM-534(T) = LMG 28481(T) = CCM 8544(T) = CIP 110828(T)) is proposed.
从田间种植的玉米植株根际分离出的一株略带黄色色素的菌株（JM-534(T)），采用多相分类法对其进行分类鉴定。观察到该分离株的细胞呈杆状，革兰氏染色阴性。比较16S rRNA基因序列分析表明，该分离株与乳酸金黄杆菌（98.9%）、约氏金黄杆菌和产吲哚金黄杆菌（均为98.7%）以及内脏金黄杆菌（98.6%）的序列相似性最高。与所有其他金黄杆菌属物种的序列相似性均为98.5%或更低。该菌株的脂肪酸分析结果显示出一种典型的金黄杆菌属模式，主要由脂肪酸C15:0异戊酸、C15:0异戊酸2-OH、C17:1异戊酸ω9c和C17:0异戊酸3-OH组成。与乳酸金黄杆菌、约氏金黄杆菌、内脏金黄杆菌和产吲哚金黄杆菌的模式菌株进行DNA-DNA杂交，结果值低于70%。基因组指纹图谱显示，该分离株与这些物种的模式菌株有很大差异。鉴别性生化和化学分类学特性表明，分离株JM-534(T)代表一个新物种，为此提出了根际金黄杆菌新种（Chryseobacterium rhizoplanae sp. nov.）（模式菌株JM-534(T) = LMG 28481(T) = CCM 8544(T) = CIP 110828(T)）。
