Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Robert Koch-Institut, Bereich Wernigerode, Wernigerode, Germany.
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1419-1427. doi: 10.1099/ijs.0.058933-0. Epub 2014 Jan 21.
Two yellow-pigmented bacterial strains (100(T) and C26(T)), showing 98.4 % 16S rRNA gene sequence similarity to each other and isolated from a chicken in Germany and as a contaminant from an agar plate of a rhizosphere sample in Alabama, were studied by using a polyphasic taxonomic approach. Cells of both isolates were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequences of the two organisms with the sequences of the type strains of the most closely related species of the genus Chryseobacterium showed the highest sequence similarities of strains 100(T) and C26(T) to the type strains of Chryseobacterium joostei (respectively 97.5 and 98.2 %), C. viscerum (96.6, 97.8 %), C. gleum (97.1, 97.7 %), C. arthrosphaerae (97.3%, 97.7 %), C. indologenes (97.2, 97.7 %), C. tructae (96.6, 97.6 %), C. jejuense (97.0, 97.6 %) and C. oncorhynchi (96.3, 97.5 %); 16S rRNA gene sequence similarities to members of all other species of the genus Chryseobacterium were below 97.5 %. The fatty acid profiles of both strains consisted of the major fatty acids iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C17 : 1ω9c and iso-C17 : 0 3-OH, but also showed slight differences (absence or presence of C16 : 0 3-OH and iso-C15 : 1 F). DNA-DNA hybridizations between the two strains and between the novel strains and the type strains of C. joostei, C. indologenes, C. jejuense, C. tructae and C. viscerum resulted in relatedness values clearly below 70 %. These DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed that both strains 100(T) and C26(T) represent novel species, for which the names Chryseobacterium gallinarum sp. nov. (type strain 100(T) = LMG 27808(T) = CCM 8493(T)) and Chryseobacterium contaminans sp. nov. (type strain C26(T) = LMG 27810(T) = CCM 8492(T)) are proposed.
从德国的一只鸡中分离到的两株黄色着色的细菌（100（T）和 C26（T）），与阿拉巴马州根际样本琼脂平板上的一种污染物显示出 98.4%的 16S rRNA 基因序列相似性，通过多相分类学方法进行了研究。两个分离株的细胞均为杆状，革兰氏阴性染色。将这两种生物的 16S rRNA 基因序列与 Chryseobacterium 属最密切相关的物种的模式株序列进行比较，显示菌株 100（T）和 C26（T）与 Chryseobacterium joostei（分别为 97.5%和 98.2%）、C. viscerum（96.6%、97.8%）、C. gleum（97.1%、97.7%）、C. arthrosphaerae（97.3%、97.7%）、C. indologenes（97.2%、97.7%）、C. tructae（96.6%、97.6%）、C. jejuense（97.0%、97.6%）和 C. oncorhynchi（96.3%、97.5%）的模式株的序列相似性最高；与该属所有其他种的成员的 16S rRNA 基因序列相似性均低于 97.5%。两个菌株的脂肪酸图谱均由主要脂肪酸 iso-C15：0、特征 3（iso-C15：0 2-OH 和/或 C16：1ω7c）、iso-C17：1ω9c 和 iso-C17：0 3-OH 组成，但也存在细微差异（C16：0 3-OH 和 iso-C15：1 F 的存在或缺失）。两个菌株之间以及两个新菌株与 Chryseobacterium joostei、C. indologenes、C. jejuense、C. tructae 和 C. viscerum 的模式株之间的 DNA-DNA 杂交导致相关性值明显低于 70%。这些 DNA-DNA 杂交结果以及区分的生化和化学分类学特性表明，两个菌株 100（T）和 C26（T）均代表新种，分别命名为 Chryseobacterium gallinarum sp. nov.（模式株 100（T）=LMG 27808（T）=CCM 8493（T））和 Chryseobacterium contaminans sp. nov.（模式株 C26（T）=LMG 27810（T）=CCM 8492（T））。
