Kim Myung Kyum, Im Wan-Taek, Shin Yong Kook, Lim Ju Hyoung, Kim Sung-Hye, Lee Byung Chul, Park Mi-Yeoun, Lee Ki Young, Lee Sung-Taik
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kuseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Division of Genome Resources Bank and Reservation, National Genome Research Institute, Korean National Institute of Health, Seoul 122-701, Republic of Korea.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2319-2324. doi: 10.1099/ijs.0.02998-0.
Gram-negative, non-spore-forming, rod-shaped, yellow-pigmented bacteria isolated from a freshwater stream in Korea were investigated to determine their taxonomic position. Complete 16S rRNA gene sequence analysis indicated that the organisms should be placed in the Chryseobacterium-Bergeyella-Riemerella branch in the family Flavobacteriaceae. Phylogenetically, the strains were most closely related to Chryseobacterium balustinum ATCC 33487(T) and Chryseobacterium scophthalmum LMG 13028(T) (94.3 and 94.1 % 16S rRNA gene sequence similarity, respectively) and they clustered on a separate well-supported branch. The strains contained menaquinone MK-6 as the predominant respiratory quinone and showed higher G+C contents (41.7 mol%) than other species in the Chryseobacterium-Bergeyella-Riemerella branch and i-C(15 : 0) as a major fatty acid (47-52 %). The phylogenetic distances from any species with validly published names and their phenotypic properties confirmed that the strains constitute a separate species in a new genus, for which the name Kaistella koreensis gen. nov., sp. nov. is proposed (type strain Chj707(T)=KCTC 12107(T)=IAM 15050(T)).
从韩国一条淡水溪流中分离出的革兰氏阴性、无芽孢、杆状、产黄色色素的细菌，对其分类地位进行了研究。完整的16S rRNA基因序列分析表明，这些微生物应归入黄杆菌科的金黄杆菌-伯杰氏菌-里默氏菌分支。系统发育分析显示，这些菌株与巴氏金黄杆菌ATCC 33487(T)和眼状金黄杆菌LMG 13028(T)关系最为密切（16S rRNA基因序列相似性分别为94.3%和94.1%），它们聚集在一个单独的、支持度良好的分支上。这些菌株以甲基萘醌MK-6作为主要呼吸醌，并且在金黄杆菌-伯杰氏菌-里默氏菌分支中显示出比其他物种更高的G+C含量（41.7 mol%），以i-C(15 : 0)作为主要脂肪酸（47-52%）。与任何有效发表名称的物种的系统发育距离及其表型特征证实，这些菌株构成了一个新属中的一个单独物种，为此提出新属名Kaistella koreensis gen. nov., sp. nov.（模式菌株Chj707(T)=KCTC 12107(T)=IAM 15050(T)）。
