Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, United States.
Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, United States.
Methods Cell Biol. 2023;173:77-89. doi: 10.1016/bs.mcb.2022.07.007. Epub 2022 Sep 2.
The plasma membrane is a fluid structure that protects cells as one of their first barriers and actively participates in numerous biological processes in many ways including through distinct membrane sub-regions. For immunological cells, highly organized sub-compartments of plasma membranes are vital for them to sense and react to environmental changes. This includes a varying spectrum of lipid ordering in the plasma membrane which signifies or enables cellular functions. Thus, comprehensive analyses of the plasma membrane can facilitate understanding of important cell biological elements which include insights into immune cells. Here, we describe two methods that can be used to assess membrane lipid state at the natural killer cell immunological synapse via high-resolution live cell imaging techniques.
质膜是一种流动的结构，作为细胞的第一道屏障之一，它通过独特的膜亚区以多种方式积极参与许多生物学过程。对于免疫细胞来说，质膜的高度组织化亚区对于它们感知和对环境变化做出反应至关重要。这包括质膜中脂质有序性的变化，这种变化标志着或使细胞功能成为可能。因此，对质膜的全面分析可以促进对包括免疫细胞在内的重要细胞生物学元素的理解。在这里，我们描述了两种可以通过高分辨率活细胞成像技术在自然杀伤细胞免疫突触中评估膜脂质状态的方法。
