颗粒鞘氨醇单胞菌新种，一种在变形菌门α-4亚类鞘脂单胞菌科中产生β-葡萄糖苷酶的细菌。

Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria.

作者信息

Kim Myung Kyum, Im Wan-Taek, Ohta Hiroyuki, Lee Myungjin, Lee Sung-Taik

机构信息

Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea.

出版信息

J Microbiol. 2005 Apr;43(2):152-7.

PMID:15880090

Abstract

Strain Kw07T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater. 16S rRNA gene sequence analysis revealed that strain Kw07T belongs to the alpha-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033T(97.8%). Chemotaxonomic data revealed that strain Kw07T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C18:1 omega7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07T represents a distinct species. Based on these data, Kw07T (=KCTC 12209T=NBRC 100800T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.

摘要

菌株Kw07T是一种革兰氏阴性、无芽孢、杆状细菌，从用于处理啤酒厂废水的上流式厌氧污泥床（UASB）生物反应器中的颗粒中分离得到。16S rRNA基因序列分析表明，菌株Kw07T属于变形菌门的α-4亚类，序列相似性最高的是大谷鞘氨醇单胞菌IFO 15033T（97.8%）。化学分类学数据表明，菌株Kw07T具有以主要化合物Q-10、主要脂肪酸C18:1 ω7c和鞘脂为特征的醌系统，所有这些都证实了我们将该菌株归为鞘氨醇单胞菌属的分类。DNA-DNA杂交以及生理生化测试结果清楚地表明，菌株Kw07T代表一个独特的物种。基于这些数据，Kw07T（=KCTC 12209T=NBRC 100800T）应被分类为一个新的鞘氨醇单胞菌物种的模式菌株，为此已提议将其命名为颗粒鞘氨醇单胞菌（Sphingopyxis granuli sp. nov.）。