Glaeser Stefanie P, Kämpfer Peter, Busse Hans-Jürgen, Langer Stefan, Glaeser Jens
Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):323-30. doi: 10.1099/ijs.0.65852-0.
A yellow- to orange-pigmented, Gram-negative, rod-shaped, motile and non-spore-forming bacterium, strain FSW06-204d(T), was isolated from subsurface water of the acidic bog lake, Lake Grosse Fuchskuhle (Brandenburg, Germany). Optimum growth of this strain occurred over a pH range from 5.5 to 6.0 and the growth rate strongly decreased at pH values above 6.5. In addition, the strain exhibited a low tolerance towards NaCl and grew only at a NaCl concentration of up to 0.5 %. 16S rRNA gene sequence analysis of strain FSW06-204d(T) showed the highest sequence similarity to Novosphingobium hassiacum W-51(T) (96.7 %) and formed a distinct cluster with Novosphingobium nitrogenifigens DSM 19370(T) (96.4 %) within the genus Novosphingobium. Strain FSW06-204d(T) shared a 21 bp signature gap with the latter species, a feature that is absent in all other members of the family Sphingomonadaceae. DNA-DNA hybridization of strain FSW06-204d(T) and N. nitrogenifigens DSM 19370(T) showed a low relatedness value of 24 % (reciprocal: 39 %). The major respiratory quinone was ubiquinone Q-10 (91 %) and the predominant fatty acid was C(18 : 1)omega7c (43.3 %). Two characteristic 2-hydroxy fatty acids, C(14 : 0) 2-OH (8.1 %) and C(15 : 0) 2-OH (6.5 %), were abundant. Polar lipids consisted mainly of phosphatidyldimethylethanolamine and phosphatidylethanolamine; however, only moderate amounts of sphingoglycolipids were present and phosphatidylcholine was lacking. Characterization by 16S rRNA gene sequence, physiological features, pigment analysis and polyamine, ubiquinone, polar lipid and fatty acid contents revealed that strain FSW06-204d(T) represents a novel species of the genus Novosphingobium within the class Alphaproteobacteria. The name Novosphingobium acidiphilum sp. nov. is proposed for this acidophilic and salt-sensitive species with the type strain FSW06-204d(T) (=DSM 19966(T)=CCM 7496(T)=CCUG 55538(T)).
从酸性沼泽湖大富克斯库勒湖（德国勃兰登堡）的地下水分离出一株黄色至橙色色素沉着、革兰氏阴性、杆状、具运动性且不形成芽孢的细菌，菌株FSW06 - 204d(T)。该菌株的最适生长pH范围为5.5至6.0，在pH值高于6.5时生长速率显著下降。此外，该菌株对NaCl耐受性较低，仅在NaCl浓度高达0.5%时生长。菌株FSW06 - 204d(T)的16S rRNA基因序列分析显示，其与哈西诺沃斯氏菌W - 51(T)的序列相似性最高（96.7%），并在新鞘氨醇菌属内与嗜氮新鞘氨醇菌DSM 19370(T)（96.4%）形成一个独特的聚类。菌株FSW06 - 204d(T)与后一个种有一个21 bp的特征性间隔区，这是鞘脂单胞菌科所有其他成员所没有的特征。菌株FSW06 - 204d(T)与嗜氮新鞘氨醇菌DSM 19370(T)的DNA - DNA杂交显示相关性较低，为24%（ reciprocal：39%）。主要呼吸醌为泛醌Q - 10（91%），主要脂肪酸为C(18 : 1)ω7c（43.3%）。两种特征性的2 - 羟基脂肪酸，C(14 : 0) 2 - OH（8.1%）和C(15 : 0) 2 - OH（6.5%）含量丰富。极性脂质主要由磷脂酰二甲基乙醇胺和磷脂酰乙醇胺组成；然而，仅存在适量的鞘糖脂，且缺乏磷脂酰胆碱。通过16S rRNA基因序列、生理特征、色素分析以及多胺、泛醌、极性脂质和脂肪酸含量的表征表明，菌株FSW06 - 204d(T)代表了α - 变形菌纲新鞘氨醇菌属的一个新种。为此嗜酸性且对盐敏感的物种提出新名称嗜酸新鞘氨醇菌（Novosphingobium acidiphilum sp. nov.），模式菌株为FSW06 - 204d(T)（=DSM 19966(T)=CCM 7496(T)=CCUG 55538(T)）。
