Van Houdt Rob, Moons Pieter, Jansen An, Vanoirbeek Kristof, Michiels Chris W
Laboratory of Food Microbiology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
FEMS Microbiol Lett. 2005 May 15;246(2):265-72. doi: 10.1016/j.femsle.2005.04.016.
Recently, we isolated from a raw vegetable processing line a Serratia strain with strong biofilm-forming capacity and which produced N-acyl-L-homoserine lactones (AHLs). Within the Enterobacteriaceae, strains of the genus Serratia are a frequent cause of human nosocomial infections; in addition, biofilm formation is often associated with persistent infections. In the current report, we describe the detailed characterization of the isolate using a variety of genotypic and phenotypic criteria. Although the strain was identified as Serratia plymuthica on the basis of its small subunit ribosomal RNA (16S rRNA) gene sequence, it differed from the S. plymuthica type strain in production of pigment and antibacterial compounds, and in AHL production profile. Nevertheless, the identification as S. plymuthica could be confirmed by gyrB phylogeny and DNA:DNA hybridization.
最近，我们从一条生蔬菜加工生产线中分离出一株具有很强生物膜形成能力且能产生N-酰基-L-高丝氨酸内酯（AHLs）的沙雷氏菌菌株。在肠杆菌科中，沙雷氏菌属的菌株是人类医院感染的常见病因；此外，生物膜形成通常与持续性感染有关。在本报告中，我们使用多种基因型和表型标准描述了该分离株的详细特征。尽管根据其小亚基核糖体RNA（16S rRNA）基因序列该菌株被鉴定为普城沙雷氏菌，但在色素和抗菌化合物的产生以及AHL产生谱方面，它与普城沙雷氏菌模式菌株有所不同。然而，通过gyrB系统发育分析和DNA:DNA杂交可以确认其为普城沙雷氏菌。
