Yanardag R, Ozsoy-Sacan O, Bolkent S, Orak H, Karabulut-Bulan O
Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, Turkey.
Hum Exp Toxicol. 2005 Mar;24(3):129-35. doi: 10.1191/0960327104ht507oa.
Metformin is a biguanide derivate used as an oral hypoglycaemic drug in diabetics. The aim of this study was to examine the histological and biochemical effects of metformin in streptozotocin (STZ)-treated rats. The animals were rendered diabetic by intraperitoneal injection of 65 mg/kg STZ. Fourteen days later, metformin was given at 25 mg/kg by gavage, daily for 28 days, to STZ-diabetic rats and a control group. In the STZ-diabetic group, some degenerative changes were observed by light microscopic examination. But the degenerative changes were decreased in the STZ-diabetic group given metformin. In the STZ-diabetic group, blood glucose levels, serum alanine and aspartate transaminase (ALT and AST) activities, total lipid levels, and sodium and potassium levels increased, while body weight, serum magnesium levels and liver glutathione (GSH) levels decreased. In the STZ-diabetic group given metformin, blood glucose levels, serum ALT and AST activities, total lipid, and sodium and potassium levels decreased, and liver GSH and serum magnesium levels increased. As a result of all the morphological and biochemical findings obtained, it was concluded that metformin has a protective effect against the hepatotoxicity produced by STZ diabetes.
二甲双胍是一种双胍类衍生物，用作糖尿病患者的口服降糖药。本研究的目的是研究二甲双胍对链脲佐菌素（STZ）处理的大鼠的组织学和生化影响。通过腹腔注射65mg/kg STZ使动物患糖尿病。14天后，将二甲双胍以25mg/kg的剂量通过灌胃给予STZ糖尿病大鼠和对照组，每天一次，持续28天。在STZ糖尿病组中，通过光学显微镜检查观察到一些退行性变化。但给予二甲双胍的STZ糖尿病组中退行性变化减少。在STZ糖尿病组中，血糖水平、血清丙氨酸和天冬氨酸转氨酶（ALT和AST）活性、总脂质水平以及钠和钾水平升高，而体重、血清镁水平和肝脏谷胱甘肽（GSH）水平降低。在给予二甲双胍的STZ糖尿病组中，血糖水平、血清ALT和AST活性、总脂质以及钠和钾水平降低，肝脏GSH和血清镁水平升高。根据所获得的所有形态学和生化结果，得出结论：二甲双胍对STZ糖尿病产生的肝毒性具有保护作用。
