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日本海参（刺参）二十个微卫星位点的分离与特征分析

Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (Stichopus japonicus).

作者信息

Kanno Manami, Li Qi, Kijima Akihiro

机构信息

Integrated Center of Field Sciences, Graduate School of Agricultural Science, Tohoku University, Onagawa, Miyagi, 986-2242, Japan.

出版信息

Mar Biotechnol (NY). 2005 May-Jun;7(3):179-83. doi: 10.1007/s10126-004-0006-3. Epub 2005 May 26.

DOI:10.1007/s10126-004-0006-3
PMID:15906112
Abstract

Twenty microsatellite markers were first developed from the Japanese sea cucumber Stichopus japonicus using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. The high variability of the microsatellite markers identified in this study will make them excellent tools for genetic analyses of S. japonicus.

摘要

首先使用富集方案从日本海参刺参中开发出20个微卫星标记。在所检测的群体中，这20个微卫星位点中有19个位点具有多态性。在这些多态性位点上，每个位点的等位基因数量从2到15不等，观察到的杂合度范围为0.03至0.97，这大大高于先前在等位酶中发现的杂合度。本研究中鉴定出的微卫星标记的高变异性将使其成为刺参遗传分析的优秀工具。

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