Liao Meijie, Wang Yingeng, Rong Xiaojun, Zhang Zheng, Li Bin, Wang Lan, Chen Guiping
Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China; E-Mails:
Int J Mol Sci. 2011;12(9):5862-70. doi: 10.3390/ijms12095862. Epub 2011 Sep 9.
Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus ranged from 0.03 to 0.89 with an average of 0.64. Cross-species amplification was also conducted in Parastichopus parvimensis collected from the United States and Pathallus mollis collected from Peru. The result showed that 17 loci amplified Parastichopus parvimensis DNAs while only 4 loci could amplify Pathallus mollis DNAs. All of the polymorphic markers would be useful for future genetic breeding and the assessment of genetic variation within sea cucumbers.
开发了20个海参微卫星DNA标记，并用于研究从中国采集的60只野生刺参个体的多态性。结果显示所有标记均具有多态性。在20个位点共检测到164个等位基因。每个位点的等位基因数从3到17不等，平均为8.2，每个位点的期望杂合度范围从0.03到0.89，平均为0.64。还对从美国采集的小刺参和从秘鲁采集的软帕海参进行了跨物种扩增。结果表明，17个位点能扩增小刺参的DNA，而只有4个位点能扩增软帕海参的DNA。所有这些多态性标记将有助于未来的遗传育种以及海参遗传变异评估。
