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能够使松柏醛和芥子醛糖基化的拟南芥糖基转移酶的鉴定与特性分析

Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde.

作者信息

Lim Eng-Kiat, Jackson Rosamond G, Bowles Dianna J

机构信息

CNAP, Department of Biology, University of York, UK.

出版信息

FEBS Lett. 2005 May 23;579(13):2802-6. doi: 10.1016/j.febslet.2005.04.016. Epub 2005 Apr 25.

Abstract

This study describes the substrate recognition profile of UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols.

摘要

本研究描述了拟南芥UDP - 葡萄糖:糖基转移酶UGT72E1的底物识别谱,它是糖基转移酶一个分支的第三个成员,能够结合木质素单体及相关代谢物。数据表明,与两个密切相关的UGT72E2和UGT72E3不同,UGT72E1对芥子醛和松柏醛具有特异性。对UGT72E1的生化特性进行了表征,并与UGT72E2的生化特性进行了比较,UGT72E2能够将醛以及松柏醇和芥子醇糖基化。

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