血清缺糖转铁蛋白测定在酒精性肝病诊断中的应用
Determination of serum carbohydrate-deficient transferrin in the diagnosis of alcoholic liver disease.
作者信息
Liu You-Shi, Xu Geng-Yun, Cheng Dong-Qing, Li You-Ming
机构信息
Department of Gastroenterology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
出版信息
Hepatobiliary Pancreat Dis Int. 2005 May;4(2):265-8.
BACKGROUND
Alcoholic liver disease (ALD) is a serious and potentially fetal consequence of alcohol use. The diagnosis of ALD is based on alcohol consumption, physical signs and symptoms, and laboratory tests. The aim of the present study was to assess the reliability of carbohydrate-deficient transferrin (CDT) in the diagnosis of ALD.
METHODS
According to the diagnostic criteria for ALD by the Chinese Medical Association in 1995, 76 patients with ALD, 55 patients with alcoholism, 32 patients with nonalcoholic liver disease (NALD), and 27 healthy subjects (controls) were studied. Serum CDT was assayed by isoelectric focusing immunofixation and Comassie blue staining. The levels of alamine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyltransferase (GGT) were also examined.
RESULTS
The positive rate of CDT in the patients with ALD was 93.4%(71/76), which was higher than that in those with alcoholism (52.7%, 29/55, P<0.001), in those with NALD(9.4%, 3/32, P<0.001), and in healthy controls, respectively. The sensitivity and specificity of CDT for ALD was 93.4% and 71.9%, respectively.
CONCLUSION
CDT may help diagnose alcoholic liver disease.
背景
酒精性肝病（ALD）是饮酒的一种严重且可能危及生命的后果。ALD的诊断基于饮酒情况、体征和症状以及实验室检查。本研究的目的是评估缺糖转铁蛋白（CDT）在ALD诊断中的可靠性。
方法
根据中华医学会1995年制定的ALD诊断标准，对76例ALD患者、55例酒精中毒患者、32例非酒精性肝病（NALD）患者和27名健康受试者（对照组）进行研究。采用等电聚焦免疫固定和考马斯亮蓝染色法检测血清CDT。同时检测丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和γ-谷氨酰转移酶（GGT）水平。
结果
ALD患者中CDT的阳性率为93.4%（71/76），分别高于酒精中毒患者（52.7%，29/55，P<0.001）、NALD患者（9.4%，3/32，P<0.001）和健康对照组。CDT对ALD的敏感性和特异性分别为93.4%和71.9%。
结论
CDT可能有助于酒精性肝病的诊断。
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