Kimura Hiroshi, Ihira Masaru, Enomoto Yoshihiro, Kawada Jun-ichi, Ito Yoshinori, Morishima Tsuneo, Yoshikawa Tetsushi, Asano Yoshizo
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.
Med Microbiol Immunol. 2005 Aug;194(4):181-5. doi: 10.1007/s00430-005-0242-9. Epub 2005 May 21.
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.
环介导等温扩增（LAMP）是一种新型核酸扩增方法，可在等温条件下以高特异性、高效率和高速度扩增DNA。为评估LAMP用于诊断单纯疱疹病毒（HSV）中枢神经系统感染的效用，我们使用先前经巢式PCR检测的样本，将LAMP方法与实时PCR进行了比较。我们检测了69例疑似患有中枢神经系统HSV感染患者的脑脊液（CSF）样本。实时PCR分析和巢式PCR检测结果完全一致。以巢式PCR为标准时，LAMP的敏感性为81%，特异性为100%，阳性预测值为100%，阴性预测值为90%。尽管要广泛应用还需进一步改进，但LAMP方法可能适用于中枢神经系统HSV感染的诊断。
