Ayi Fanou Lucie, Mobio Théophile A, Creppy Edmond E, Fayomi Benjamin, Fustoni Silvia, Møller Peter, Kyrtopoulos Soterios, Georgiades Panos, Loft Steffen, Sanni Ambaliou, Skov Henrik, Ovrebø Steinar, Autrup Herman
Laboratoire de Biochimie et de Biologie Moleculaire, Faculte des Sciences et Techniques, Universite d'Abomey-Calavi, Republique du Benin. 04 BP 0320 Cotonou, Republique du Benin.
Sci Total Environ. 2006 Apr 1;358(1-3):85-96. doi: 10.1016/j.scitotenv.2005.03.025.
Exposure to genotoxic compounds present in ambient air has been studied in Cotonou, Benin, a city where two-stroke motorbikes are the major form of transportation and gasoline quality is poor. Personal monitoring and biomarkers were used to assess the exposure. Non-smoking taxi-moto drivers (city) and village residents were the study subjects. Benzene exposure was significantly higher in the city, as compared to the village (76.0+/-26.8 microg/m(3) versus 3.4+/-3.0, p=0.0004). Urinary excretion of benzene and S-phenylmercapturic acid (S-PMA) were also highest in subjects living in the city, whereas 1-hydroxypyrene was not different. The level of total polycyclic aromatic hydrocarbons (PAHs), associated with particles, ranged from 76.21 to 103.23 in Cotonou versus 1.55 ng/m(3) for the village. Determination of DNA damage in lymphocytes showed that subjects from the city had elevated number of lesions compared to subjects in the village in terms of bulky DNA adducts, 8-hydroxy-2'-deoxyguanosine and 5-methylcytosine, whereas DNA fragmentations analysed by alkaline gel electrophoresis was not different between the subjects. In conclusion, this study shows that air pollution is pronounced in Cotonou, Bénin and is associated with elevated levels of DNA damage in residents of the city compared to people living in a non-polluted rural village.
在贝宁的科托努，人们对环境空气中存在的遗传毒性化合物暴露情况进行了研究。在这个城市，两轮摩托车是主要的交通方式，汽油质量较差。研究采用个人监测和生物标志物来评估暴露情况。研究对象为不吸烟的出租车摩托车司机（城市）和乡村居民。与乡村相比，城市中的苯暴露显著更高（76.0±26.8微克/立方米对3.4±3.0，p = 0.0004）。居住在城市的人群中，苯和S - 苯巯基尿酸（S - PMA）的尿排泄量也最高，而1 - 羟基芘则无差异。与颗粒物相关的总多环芳烃（PAHs）水平，在科托努为76.21至103.23，而乡村为1.55纳克/立方米。淋巴细胞DNA损伤测定表明，就大分子DNA加合物、8 - 羟基 - 2'-脱氧鸟苷和5 - 甲基胞嘧啶而言，城市受试者的损伤数量高于乡村受试者，而通过碱性凝胶电泳分析的DNA片段化在两组受试者之间无差异。总之，本研究表明，贝宁的科托努空气污染严重，与未受污染的乡村居民相比，城市居民的DNA损伤水平升高。
