Flores J M, Spivak M, Gutiérrez I
Centro Andaluz de Apicultura Ecológica, Universidad de Córdoba, Campus Universitario de Rabanales, 14071 Córdoba, Spain.
Vet Microbiol. 2005 Jun 15;108(1-2):141-4. doi: 10.1016/j.vetmic.2005.03.005. Epub 2005 Apr 26.
Chalkbrood disease in honeybees (Apis mellifera L.) is caused by an infection with Ascosphaera apis. Disease expression requires the consumption of fungal spores and a predisposing condition in the susceptible brood. A. apis spores within sheets of wax foundation could be a source of inoculum leading to chalkbrood, but it is also possible that these spores remain confined in the wax and do not contribute to disease. We have resolved this topic by chilling susceptible brood within wax combs built on contaminated foundation (using treatments of spores from 1 mummy and spores from 10 mummies) versus uncontaminated foundation. We found significantly higher levels of chalkbrood in brood exposed to the higher dosage. Our results demonstrate that foundation wax contaminated with spores of A. apis spores may be a source of chalkbrood in honeybee colonies.
蜜蜂（西方蜜蜂）的白垩病是由蜜蜂球囊菌感染引起的。疾病的表现需要消耗真菌孢子以及易感幼虫存在易发病的条件。巢础片内的蜜蜂球囊菌孢子可能是导致白垩病的接种源，但这些孢子也有可能被困在蜡中，不会引发疾病。我们通过将建在受污染巢础（分别使用来自1个木乃伊的孢子处理和来自10个木乃伊的孢子处理）与未受污染巢础上的蜡脾中的易感幼虫进行冷藏，解决了这个问题。我们发现暴露于较高剂量孢子的幼虫中白垩病的发生率显著更高。我们的结果表明，被蜜蜂球囊菌孢子污染的巢础蜡可能是蜜蜂蜂群中白垩病的一个来源。
