Pinent M, Bladé M C, Salvadó M J, Arola L, Hackl H, Quackenbush J, Trajanoski Z, Ardévol A
Department of Biochemistry and Biotechnology, Rovira i Virgili University, Tarragona, Spain.
Int J Obes (Lond). 2005 Aug;29(8):934-41. doi: 10.1038/sj.ijo.0802988.
Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-gamma (PPARgamma) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARgamma2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells.
We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h.
Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique).
Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE.
These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.
我们团队之前关于葡萄籽原花青素提取物（GSPE）对脂肪代谢影响的研究结果表明，过氧化物酶体增殖物激活受体γ（PPARγ）在GSPE对脂肪细胞的脂解作用中起核心作用。由于PPARγ2是脂肪细胞分化过程的主要调节因子，我们研究了GSPE是否会影响3T3-L1细胞的脂肪生成。
我们在分化过程中用GSPE处理3T3-L1细胞24小时，进行了时间点筛选。
GSPE处理后的分化标志物和差异基因表达（使用微阵列技术）。
在分化开始时进行24小时的GSPE处理可降低脂肪特异性标志物，并使前脂肪细胞标志物前脂肪细胞因子-1（Pref-1）的表达维持在显著升高的水平。在其他时间点未发现这些效应。对分化早期GSPE处理后的基因表达进行微阵列分析显示，基因表达谱发生了改变，其中细胞周期和生长相关基因被GSPE下调。
这些结果表明，GSPE主要在分化诱导阶段影响脂肪生成，并且原花青素可能在阻碍脂肪细胞形成方面具有新作用。
