Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
Biomed Pharmacother. 2017 Feb;86:292-296. doi: 10.1016/j.biopha.2016.12.019. Epub 2016 Dec 21.
Procyanidins have lipolysis effect on adipose metabolism, but the underlying mechanism is not fully understood. The aim of present study was to examine the effect of grape seed procyanidin B2 (GSP) on the adipogenic differentiation of 3T3-L1 preadipocyte cell line and investigate the underlying mechanism. The results showed that GSP treatment significantly reduced the intracellular lipid accumulation in induced 3T3-L1 cells by targeting miR-483-5p as well as peroxisome proliferator-activated receptor γ (PPARγ). In addition, our results revealed that overexpression of miR-483-5p increased adipogenic differentiation, while inhibition of miR-483-5p reduced the lipid accumulation by suppressing the adipogenic differentiation. Moreover, overexpression of miR-483-5p could reverse GSP's inhibition of adipocyte differentiation as well as increase the level of PPARγ. These results demonstrate that GSP inhibits adipogenesis by targeting PPARγ and suggest this effect might be mediated by miR-483-5p.
原花青素对脂肪代谢有脂解作用，但作用机制尚不完全清楚。本研究旨在探讨葡萄籽原花青素 B2 (GSP) 对 3T3-L1 前脂肪细胞系成脂分化的影响，并探讨其作用机制。结果表明，GSP 通过靶向 miR-483-5p 以及过氧化物酶体增殖物激活受体 γ (PPARγ)，显著减少了诱导的 3T3-L1 细胞内的脂滴积累。此外，我们的结果表明，miR-483-5p 的过表达增加了成脂分化，而抑制 miR-483-5p 通过抑制成脂分化减少了脂滴积累。此外，miR-483-5p 的过表达可以逆转 GSP 对脂肪细胞分化的抑制作用，并增加 PPARγ 的水平。这些结果表明，GSP 通过靶向 PPARγ 抑制脂肪生成，并提示这种作用可能是由 miR-483-5p 介导的。
