Row Kyung Ho, Jin Yinzhe
Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, South Korea.
Bioresour Technol. 2006 Mar;97(5):790-3. doi: 10.1016/j.biortech.2005.04.001.
Catechin compounds from Korean green tea as potential sources of anticancer and antioxidant components were target materials in this work. The methodologies of solvent extraction and partition were utilized to recover catechin compounds from green tea. The optimum experimental condition was obtained by optimizing operating factors, such as, the extraction solvent, extraction time and operating temperature. After extracting the green tea with water at 80 degrees C for 40 min, the extract was partitioned with water/chloroform, which was best suited to remove caffeine impurity from the extract. Further, the resulting extract was partitioned water/ethyl acetate to deeply purify the catechin compounds of EGC, EC, EGCG and ECG. The experimental result in this work could be extended to preparative HPLC to obtain EGCG on commercial scale.
来自韩国绿茶的儿茶素化合物作为抗癌和抗氧化成分的潜在来源是本研究的目标材料。采用溶剂萃取和分配方法从绿茶中回收儿茶素化合物。通过优化操作因素，如萃取溶剂、萃取时间和操作温度，获得了最佳实验条件。在80℃用水萃取绿茶40分钟后，萃取物用水/氯仿进行分配，这最适合从萃取物中去除咖啡因杂质。此外，将所得萃取物用水/乙酸乙酯进行分配，以深度纯化表没食子儿茶素（EGC）、儿茶素（EC）、表没食子儿茶素没食子酸酯（EGCG）和儿茶素没食子酸酯（ECG）的儿茶素化合物。本研究的实验结果可扩展到制备型高效液相色谱法，以商业规模获得EGCG。
