Kleinrichert Kody, Alappat Bindhu
Department of Chemistry, Saint Xavier University, Chicago, IL 60655, USA.
Antioxidants (Basel). 2019 Jan 1;8(1):13. doi: 10.3390/antiox8010013.
Though the pathogenesis of Alzheimer's Disease (AD) is not completely elucidated, it is generally accepted that the aggregation of toxic amyloid-β (Aβ) protein fibrils plays a major role in the disease's onset and progression. Various phytoceutical compounds have been shown to attenuate Aβ toxicity and disrupt its aggregation, including various types of polyphenolic compounds. These polyphenolic compounds have also been found to demonstrate potent antioxidant activity, which may contribute to their anti-amyloidogenic properties. This study compares three plants, traditionally used for numerous medicinal purposes in Asian countries, including: (Turmeric), (Green Tea), and (Sweet Broomweed). Antioxidant effects of the crude, polyphenol rich phytoceutical extracts from these plants were analyzed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ability of these extracts to prevent Aβ fibril formation was then carried out in order to establish a relationship between antioxidant activity and Aβ aggregation. A positive correlation between antioxidant efficacy and prevention of Aβ aggregation was demonstrated, indicating that antioxidant activity may play some role in preventing Aβ aggregation.
尽管阿尔茨海默病（AD）的发病机制尚未完全阐明，但人们普遍认为有毒的淀粉样β（Aβ）蛋白纤维的聚集在该疾病的发生和发展中起主要作用。各种植物源化合物已被证明可减轻Aβ毒性并破坏其聚集，包括各种类型的多酚化合物。这些多酚化合物还被发现具有强大的抗氧化活性，这可能有助于它们的抗淀粉样蛋白生成特性。本研究比较了三种在亚洲国家传统上用于多种药用目的的植物，包括：（姜黄）、（绿茶）和（甜扫帚草）。使用2,2-二苯基-1-苦基肼（DPPH）测定法分析了这些植物富含多酚的粗制植物源提取物的抗氧化作用。然后进行这些提取物防止Aβ纤维形成的能力分析，以建立抗氧化活性与Aβ聚集之间的关系。结果表明抗氧化功效与预防Aβ聚集之间存在正相关，表明抗氧化活性可能在预防Aβ聚集中发挥一定作用。
