Wu Sue-Jing, Ng Lean-Teik, Huang Yuan-Man, Lin Doung-Liang, Wang Shyh-Shyan, Huang Shan-Ney, Lin Chun-Ching
Graduate Institute of Natural Products, Kaohsiung Medical University, Taiwan.
Biol Pharm Bull. 2005 Jun;28(6):963-6. doi: 10.1248/bpb.28.963.
Physalis peruviana (PP) is a widely used medicinal herb for treating cancer, malaria, asthma, hepatitis, dermatitis and rheumatism. In this study, the hot water extract (HWEPP) and extracts prepared from different concentrations of ethanol (20, 40, 60, 80 and 95% EtOH) from the whole plant were evaluated for antioxidant activities. Results displayed that at 100 mug/ml, the extract prepared from 95% EtOH exhibited the most potent inhibition rate (82.3%) on FeCl2-ascorbic acid induced lipid peroxidation in rat liver homogenate. At concentrations 10-100 microg/ml, this extract also demonstrated the strongest superoxide anion scavenging and inhibitory effect on xanthine oxidase activities. In general, the ethanol extracts revealed a stronger antioxidant activity than alpha-tocopherol and HWEPP. Compared to alpha-tocopherol, the IC50 value of 95% EtOH PP extract was lower in thiobarbituric acid test (IC50=23.74 microg/ml vs. 26.71 microg/ml), in cytochrome c test (IC50=10.40 microg/ml vs. 13.39 microg/ml) and in xanthine oxidase inhibition test (IC50=8.97 microg/ml vs. 20.68 microg/ml). The present study concludes that ethanol extracts of PP possess good antioxidant activities, and the highest antioxidant properties were obtained from the 95% EtOH PP.
灯笼果是一种广泛用于治疗癌症、疟疾、哮喘、肝炎、皮炎和风湿病的药草。在本研究中，对从整株植物中提取的热水提取物（HWEPP）以及不同浓度乙醇（20%、40%、60%、80%和95%乙醇）制备的提取物进行了抗氧化活性评估。结果显示，在100μg/ml时，95%乙醇制备的提取物对氯化亚铁 - 抗坏血酸诱导的大鼠肝匀浆脂质过氧化表现出最强的抑制率（82.3%）。在10 - 100μg/ml浓度下，该提取物还表现出最强的超氧阴离子清除能力以及对黄嘌呤氧化酶活性的抑制作用。总体而言，乙醇提取物显示出比α - 生育酚和HWEPP更强的抗氧化活性。与α - 生育酚相比，95%乙醇灯笼果提取物在硫代巴比妥酸试验中的IC50值更低（IC50 = 23.74μg/ml对26.71μg/ml），在细胞色素c试验中（IC50 = 10.40μg/ml对13.39μg/ml）以及在黄嘌呤氧化酶抑制试验中（IC50 = 8.97μg/ml对20.68μg/ml）。本研究得出结论，灯笼果的乙醇提取物具有良好的抗氧化活性，且95%乙醇灯笼果提取物具有最高的抗氧化性能。
