Plant Molecular Genetics Laboratory, Center for Biotechnology and Bioindustry, Colombian Corporation for Agricultural Research, CORPOICA, Bogotá, Colombia.
PLoS One. 2013 Jul 3;8(7):e68500. doi: 10.1371/journal.pone.0068500. Print 2013.
The Cape gooseberry (Physalisperuviana L) is an Andean exotic fruit with high nutritional value and appealing medicinal properties. However, its cultivation faces important phytosanitary problems mainly due to pathogens like Fusarium oxysporum, Cercosporaphysalidis and Alternaria spp. Here we used the Cape gooseberry foliar transcriptome to search for proteins that encode conserved domains related to plant immunity including: NBS (Nucleotide Binding Site), CC (Coiled-Coil), TIR (Toll/Interleukin-1 Receptor). We identified 74 immunity related gene candidates in P. peruviana which have the typical resistance gene (R-gene) architecture, 17 Receptor like kinase (RLKs) candidates related to PAMP-Triggered Immunity (PTI), eight (TIR-NBS-LRR, or TNL) and nine (CC-NBS-LRR, or CNL) candidates related to Effector-Triggered Immunity (ETI) genes among others. These candidate genes were categorized by molecular function (98%), biological process (85%) and cellular component (79%) using gene ontology. Some of the most interesting predicted roles were those associated with binding and transferase activity. We designed 94 primers pairs from the 74 immunity-related genes (IRGs) to amplify the corresponding genomic regions on six genotypes that included resistant and susceptible materials. From these, we selected 17 single band amplicons and sequenced them in 14 F. oxysporum resistant and susceptible genotypes. Sequence polymorphisms were analyzed through preliminary candidate gene association, which allowed the detection of one SNP at the PpIRG-63 marker revealing a nonsynonymous mutation in the predicted LRR domain suggesting functional roles for resistance.
刺果番荔枝（Physalis peruviana L）是一种具有高营养价值和药用价值的安第斯外来水果。然而，其种植面临着重要的植物检疫问题，主要是由于病原体如尖孢镰刀菌、尾孢菌和链格孢菌等。在这里，我们使用刺果番荔枝叶片转录组来搜索编码与植物免疫相关的保守结构域的蛋白质，包括：NBS（核苷酸结合位点）、CC（卷曲螺旋）、TIR（Toll/白细胞介素-1 受体）。我们在 P. peruviana 中鉴定了 74 个与免疫相关的候选基因，这些基因具有典型的抗性基因（R-基因）结构，17 个与植物病原相关模式触发免疫（PTI）相关的类受体激酶（RLKs）候选基因，8 个（TIR-NBS-LRR 或 TNL）和 9 个（CC-NBS-LRR 或 CNL）与效应子触发免疫（ETI）基因相关的候选基因等。这些候选基因根据分子功能（98%）、生物过程（85%）和细胞成分（79%）进行了分类，使用基因本体论。一些最有趣的预测作用是与结合和转移酶活性相关的作用。我们从 74 个与免疫相关的基因（IRGs）中设计了 94 对引物，以扩增包括抗性和敏感性材料在内的六个基因型的相应基因组区域。从这些基因中，我们选择了 17 个单带扩增子，并在 14 个对 F. oxysporum 有抗性和敏感性的基因型中对它们进行了测序。通过初步候选基因关联分析对序列多态性进行了分析，在 PpIRG-63 标记中检测到一个 SNP，揭示了预测的 LRR 结构域中的非同义突变，表明该突变与抗性有关。
