Jaeckel Petra, Krauss Gudrun, Menge Sieglinde, Schierhorn Angelika, Rücknagel Peter, Krauss Gerd-Joachim
UFZ Centre for Environmental Research Leipzig-Halle in the Helmholtz Association, Department of Environmental Microbiology, Halle/Saale, Germany.
Biochem Biophys Res Commun. 2005 Jul 22;333(1):150-5. doi: 10.1016/j.bbrc.2005.05.083.
Cadmium stress response was measured at the thiol peptide level in an aquatic hyphomycete (Heliscus lugdunensis). In liquid culture, 0.1 mM cadmium increased the glutathione (GSH) content and induced the synthesis of additional thiol peptides. HPLC, electrospray ionization mass spectrometry, and Edman degradation confirmed that a novel small metallothionein as well as phytochelatin (PC2) were synthesized. The metallothionein has a high homology to family 8 metallothioneins (http://www.expasy.ch/cgi-bin/lists?metallo.txt). The bonding of at least two cadmium ions to the metallothionein was demonstrated by mass spectrometry (MALDI MS). This is the first time that simultaneous induction of metallothionein and phytochelatin accompanied by an increase in GSH level has been shown in a fungus under cadmium stress, indicating a potential function of these complexing agents for in vivo heavy metal detoxification. The method presented here should be applicable as biomarker tool.
在一种水生真菌（鲁登海氏菌）中，在硫醇肽水平上测定了镉应激反应。在液体培养中，0.1 mM镉增加了谷胱甘肽（GSH）含量并诱导了额外硫醇肽的合成。高效液相色谱、电喷雾电离质谱和埃德曼降解证实合成了一种新型小金属硫蛋白以及植物螯合肽（PC2）。该金属硫蛋白与第8族金属硫蛋白具有高度同源性（http://www.expasy.ch/cgi-bin/lists?metallo.txt）。通过质谱（基质辅助激光解吸电离质谱）证明至少两个镉离子与该金属硫蛋白结合。这是首次在镉胁迫下的真菌中显示出金属硫蛋白和植物螯合肽的同时诱导伴随着GSH水平的增加，表明这些络合剂在体内重金属解毒中的潜在功能。这里介绍的方法应可作为生物标志物工具应用。
