蜜蜂花提取物对高脂血症大鼠肝脏的保护作用：一项形态学和生物化学研究。

Protective role of Melissa officinalis L. extract on liver of hyperlipidemic rats: a morphological and biochemical study.

作者信息

Bolkent S, Yanardag R, Karabulut-Bulan Omur, Yesilyaprak B

机构信息

Istanbul University, Faculty of Science, Department of Biology, 34459-Vezneciler, Istanbul, Turkey.

出版信息

J Ethnopharmacol. 2005 Jul 14;99(3):391-8. doi: 10.1016/j.jep.2005.02.038.

DOI:10.1016/j.jep.2005.02.038

PMID:15946812

Abstract

In this study, the effects of Melissa officinalis L. extract on hyperlipidemic rats were investigated, morphologically and biochemically. The animals were fed a lipogenic diet consisting of 2% cholesterol, 20% sunflower oil and 0.5% cholic acid added to normal chow and were given 3% ethanol for 42 days. The plant extract was given by gavage technique to rats to a dose of 2 g/kg every day for 28, 14 days after experimental animals done hyperlipidemia. The degenerative changes were observed in hyperlipidemic rats, light and electron microscopically. There was a significant increase in the levels of serum cholesterol, total lipid, alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), a significant decrease in the levels of liver tissue glutathione (GSH), a significant increase in the levels of tissue lipid peroxidation (LPO) in this group. On the other hand, the administration of Melissa officinalis L. extract reduced total cholesterol, total lipid, ALT, AST and ALP levels in serum, and LPO levels in liver tissue, moreover increased glutathione levels in the tissue. As a result, it was suggested that Melissa officinalis L. extract exerted an hypolipidemic effect and showed a protective effect on the liver of hyperlipidemic rats.

摘要

本研究从形态学和生物化学方面考察了蜜蜂花提取物对高脂血症大鼠的影响。给动物喂食由添加到普通饲料中的2%胆固醇、20%向日葵油和0.5%胆酸组成的致脂饮食，并给予3%乙醇，持续42天。在实验动物形成高脂血症后，通过灌胃技术给大鼠每天给予2 g/kg剂量的植物提取物，持续28天、14天。对高脂血症大鼠进行光镜和电镜观察，发现有退行性变化。该组大鼠血清胆固醇、总脂质、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）水平显著升高，肝组织谷胱甘肽（GSH）水平显著降低，组织脂质过氧化（LPO）水平显著升高。另一方面，给予蜜蜂花提取物可降低血清中的总胆固醇、总脂质、ALT、AST和ALP水平以及肝组织中的LPO水平，此外还可提高组织中的谷胱甘肽水平。结果表明，蜜蜂花提取物具有降血脂作用，并对高脂血症大鼠的肝脏具有保护作用。