Krakowka Steven, Ellis John, McNeilly Francis, Waldner Cheryl, Allan Gordon
Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, OH 43210, USA.
J Vet Diagn Invest. 2005 May;17(3):213-22. doi: 10.1177/104063870501700301.
Tissue sets from 36 snatch-farrowed colostrum-deprived (SF/CD) and 71 Caesarian-derived gnotobiotic swine infected with porcine circovirus type 2 (PCV-2) as neonates were examined and scored for the types and tissue distribution of histologic lesions associated with this viral infection. The occurrence and severity of these lesions were correlated with qualitative and quantitative determinations of viral burden in tissues by immunohistochemistry (IHC) and tissue titrations for infectious virus, respectively. These measures were, in turn, related to 1 of 3 categories of clinical disease expressed in PCV-2-infected swine as subclinical infection, preclinical postweaning multisystemic wasting syndrome (PMWS), and clinically evident PMWS, respectively. Statistically significant (P < 0.05 to 0.001) associations between both measures of viral burden, the severity of histologic lesions and the stage of disease were obtained. Discrimination between and among categories of disease was best accomplished by a combination of IHC and histopathology. The results of this study confirm that viral burden in PCV-2-infected tissues, specifically lymphoid tissues and liver, directly correlate with severity of clinical disease expression in PCV-2 infected swine.
对36头初产时被剥夺初乳的经产仔猪（SF/CD）和71头剖腹产获得的无菌仔猪（新生时感染了2型猪圆环病毒（PCV - 2））的组织样本进行了检查，并对与这种病毒感染相关的组织学病变类型和组织分布进行了评分。这些病变的发生和严重程度分别与通过免疫组织化学（IHC）对组织中病毒载量的定性和定量测定以及对感染性病毒的组织滴定相关。反过来，这些指标又分别与PCV - 2感染猪所表现出的3种临床疾病类别之一相关，即亚临床感染、断奶前多系统消耗综合征（PMWS）和临床明显的PMWS。在病毒载量的两种测量方法、组织学病变的严重程度和疾病阶段之间获得了具有统计学意义（P < 0.05至0.001）的关联。通过IHC和组织病理学的结合能够最好地区分不同的疾病类别。本研究结果证实，PCV - 2感染组织，特别是淋巴组织和肝脏中的病毒载量，与PCV - 2感染猪临床疾病表达的严重程度直接相关。
