Puoci Francesco, Piangiolino Cristiana, Givigliano Francesco, Parisi Ortensia Ilaria, Cassano Roberta, Trombino Sonia, Curcio Manuela, Iemma Francesca, Cirillo Giuseppe, Spizzirri Umile Gianfranco, Restuccia Donatella, Muzzalupo Rita, Picci Nevio
Department of Pharmaceutical Sciences, University of Calabria, Edificio Polifunzionale, Arcavacata di Rende (CS) 87036, Italy.
Principium Europe, Via Como 45, Solaro (MI) 20020, Italy.
J Funct Biomater. 2012 May 15;3(2):361-71. doi: 10.3390/jfb3020361.
The synthesis of a novel functional biomaterial for wound healing treatment was carried out by adopting a free-radical grafting procedure in aqueous media. With this aim, ciprofloxacin (CFX) was covalently incorporated into collagen (T1C) chains employing an ascorbic acid/hydrogen peroxide redox pair as biocompatible initiator system. The covalent insertion of CFX in the polymeric chains was confirmed by FT-IR and UV analyses, while an antibacterial assay demonstrated the activity of the synthesized conjugate against Staphylococcus aureus and Escherichia coli, microorganisms that commonly infect wounds. A catechin blended conjugate was also tested in order to evaluate the ability to influence fibroblast cell growth. The observed antibacterial activity and stimulation of fibroblast growth support the applicability of CFX-T1C conjugate in wound treatment encouraging the healing process.
通过在水介质中采用自由基接枝方法合成了一种用于伤口愈合治疗的新型功能性生物材料。为此，使用抗坏血酸/过氧化氢氧化还原对作为生物相容性引发剂体系，将环丙沙星（CFX）共价结合到胶原蛋白（T1C）链中。通过傅里叶变换红外光谱（FT-IR）和紫外分析证实了CFX在聚合物链中的共价插入，而抗菌试验证明了合成的共轭物对金黄色葡萄球菌和大肠杆菌的活性，这些是常见的感染伤口的微生物。还测试了儿茶素混合共轭物以评估其影响成纤维细胞生长的能力。观察到的抗菌活性和成纤维细胞生长刺激支持了CFX-T1C共轭物在伤口治疗中的适用性，促进了愈合过程。
