Hunt John Alan, Callaghan Jill T, Sutcliffe Chris J, Morgan Rhys H, Halford Ben, Black Richard A
UK Centre for Tissue Engineering (UKCTE), University of Liverpool, Duncan Building, Daulby Street, L69 3GA, UK.
Biomaterials. 2005 Oct;26(29):5890-7. doi: 10.1016/j.biomaterials.2005.03.004. Epub 2005 Apr 15.
Improved fixation and increased longevity are still important performance criteria in the development of orthopaedic prostheses. The osseointegration of a series of implant designs made of conventional cobalt-chromium alloy was investigated, the shape of each implant being the critical variable. The shape was defined by computer-aided design with a view to maximising interdigitation of new bone with the implant. Two different process routes, conventional casting and selective laser sintering were employed, each process yielded implants that had identical surface topology but different microstructures. Hydroxyapatite (HA) was used to coat some samples by plasma spraying. Bone formation associated with each implant design was delineated through the administration of fluorescent vital dyes at three time points following their implantation into New Zealand white rabbits. After one month, specimens were harvested, resin embedded, serial sectioned and examined under fluorescent light microscopy. The amount of bone growth was quantified using image analysis. Plasma spray HA-coated samples promoted better osteogenesis and integration than uncoated samples. The extent of bone growth associated with identically shaped specimens fabricated by the SLS route was markedly greater, attributed to the microstructure of these implants.
在骨科假体的研发中，提高固定效果和延长使用寿命仍然是重要的性能标准。研究了一系列由传统钴铬合金制成的植入物设计的骨整合情况，每个植入物的形状是关键变量。通过计算机辅助设计来定义形状，目的是使新骨与植入物的相互交错最大化。采用了两种不同的工艺路线，即传统铸造和选择性激光烧结，每种工艺生产的植入物具有相同的表面拓扑结构但微观结构不同。通过等离子喷涂用羟基磷灰石（HA）对一些样品进行涂层处理。在将每种植入物设计植入新西兰白兔体内后的三个时间点，通过施用荧光活性染料来描绘与植入物相关的骨形成情况。一个月后，采集标本，用树脂包埋，进行连续切片，并在荧光显微镜下检查。使用图像分析对骨生长量进行量化。等离子喷涂HA涂层样品比未涂层样品促进了更好的骨生成和整合。通过选择性激光烧结工艺制造的形状相同的标本所伴随的骨生长程度明显更大，这归因于这些植入物的微观结构。
