Malmström Johan, Adolfsson Erik, Arvidsson Anna, Thomsen Peter
Institute for Clinical Sciences, Department of Biomaterials, Göteborg University, Sweden.
Clin Implant Dent Relat Res. 2007 Jun;9(2):79-88. doi: 10.1111/j.1708-8208.2007.00031.x.
The technique of free-form fabrication enables the production of controlled macroporous geometry inside ceramic scaffolds. Using scaffolds with identical macropore design makes it possible to study a relevant biological response linked to other specific changes of the material.
This study investigates the role of open micropores in hydroxyapatite (HA) scaffold during early bone healing to quantitatively ascertain whether microporosity in otherwise identical macroporous HA scaffolds can influence the bone response in rabbit tibia and femur at 6 weeks.
HA scaffolds (Ø: 3.8 mm) with and without microporosity were randomly installed in both cortical and trabecular bone sites of New Zealand White rabbits. The animals were sacrificed 6 weeks after surgery. Ground sections obtained from en bloc tissues containing scaffold and recipient bone were subjected to histological evaluation and histomorphometric analysis.
Microscopy showed elevated amounts of bone ingrowth and bone contact inside the microporous HA (mHA) group as compared with non-mHA.
The current study indicates that the presence of open scaffold microporosity in HA, as determined by the fabrication process, enhances the ability of ceramic scaffolds to promote bone ingrowth and bone contact.
自由成型制造技术能够在陶瓷支架内部制造出可控的大孔结构。使用具有相同大孔设计的支架可以研究与材料其他特定变化相关的生物反应。
本研究探讨羟基磷灰石（HA）支架中开放微孔在早期骨愈合过程中的作用，以定量确定在其他方面相同的大孔HA支架中的微孔性是否会影响新西兰白兔胫骨和股骨在6周时的骨反应。
将有无微孔的HA支架（直径：3.8mm）随机植入新西兰白兔的皮质骨和松质骨部位。术后6周处死动物。对包含支架和受体骨的整块组织制成的磨片进行组织学评估和组织形态计量分析。
显微镜检查显示，与无微孔HA（mHA）组相比，微孔HA（mHA）组内的骨长入和骨接触量增加。
本研究表明，通过制造工艺确定的HA支架中开放微孔的存在增强了陶瓷支架促进骨长入和骨接触的能力。
