Takemura Taichiro, Ekwalanga Michel, Bikandou Blaise, Ido Eiji, Yamaguchi-Kabata Yumi, Ohkura Sadayuki, Harada Hirotada, Takehisa Jun, Ichimura Hiroshi, Parra Henri-Joseph, Nende Monique, Mubwo Eric, Sepole Midingi, Hayami Masanori, Miura Tomoyuki
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
National Institute of Biomedical Research, Kinshasa, Democratic Republic of Congo.
J Gen Virol. 2005 Jul;86(Pt 7):1967-1971. doi: 10.1099/vir.0.80697-0.
In order to understand primate lentivirus evolution, characterization of additional simian immunodeficiency virus (SIV) strains is essential. Here, an SIV from a black mangabey (Lophocebus aterrimus) originating from the Democratic Republic of Congo was analysed phylogenetically. The monkey had cross-reactive antibodies against human immunodeficiency virus type 1 (HIV-1) and HIV-2. The viral pol region sequence was amplified by nested PCR and sequence analysis confirmed that it was related to known SIV sequences. This is the first report to characterize genetically an SIV from the monkey genus Lophocebus. Phylogenetic analysis of the pol region revealed that this novel SIV, designated SIVbkm, fell into the SIVsyk and SIVgsn virus group, containing viruses isolated from the genus Cercopithecus, and suggests that cross-species transmission has occurred between species of the genera Lophocebus and Cercopithecus.
为了了解灵长类慢病毒的进化，对更多的猴免疫缺陷病毒（SIV）毒株进行特征分析至关重要。在此，对一株源自刚果民主共和国的黑冠白睑猴（Lophocebus aterrimus）的SIV进行了系统发育分析。这只猴子具有针对1型人类免疫缺陷病毒（HIV-1）和HIV-2的交叉反应性抗体。通过巢式PCR扩增病毒的pol区域序列，序列分析证实其与已知的SIV序列相关。这是首次对来自白睑猴属猴子的SIV进行基因特征分析的报告。pol区域的系统发育分析表明，这种新型SIV被命名为SIVbkm，属于SIVsyk和SIVgsn病毒组，该组包含从猕猴属分离出的病毒，这表明白睑猴属和猕猴属之间发生了跨物种传播。
