Tomonaga K, Katahira J, Fukasawa M, Hassan M A, Kawamura M, Akari H, Miura T, Goto T, Nakai M, Suleman M
Research Center for Immunodeficiency Virus, Kyoto University, Japan.
Arch Virol. 1993;129(1-4):77-92. doi: 10.1007/BF01316886.
Forty-eight of 236 sera from seven species of African non-human primates in Kenya, including those of white-crowned mangabey monkeys (Cercocebus torquatus lunulatus) had antibodies to simian immunodeficiency viruses (SIVs). Isolates of simian lentivirus were obtained from seropositive white-crowned mangabey monkeys which are indigenous in West Africa. This virus, designated as SIVWCM, appeared morphologically similar to HIV by electron microscopy, showed Mg(2+)-dependent reverse transcriptase activity, and induced cytopathic effects in human CD4-positive cells. Western blotting analysis revealed that env products of SIVWCM cross-reacted with those of SIVAGM more strongly than with those of HIV-1 and SIVMAC, and clear hybridization bands were detected with an SIVAGM probe. For comparison of the virus sequence with those of other primate lentiviruses, part of the pol gene and the long terminal repeats (LTRs) were amplified and cloned. Sequencing showed that SIVWCM isolates were closely related to SIVAGM isolates. This study suggested that SIVAGM from the Cercopithecus genus and SIVWCM from the Cercocebus genus may be members of an SIV group that is genetically distinct from the SIV from a sooty mangabey monkey (SIVSMM) of the genus Cercocebus, to which the white-crowned mangabey monkey also belongs.
在肯尼亚采集的来自7种非洲非人类灵长类动物的236份血清样本中，有48份含有针对猿猴免疫缺陷病毒（SIV）的抗体，这些灵长类动物包括白冠白睑猴（Cercocebus torquatus lunulatus）。猿猴慢病毒分离株是从血清反应呈阳性的白冠白睑猴中获得的，这种猴子原产于西非。这种病毒被命名为SIVWCM，通过电子显微镜观察，其形态与HIV相似，具有依赖Mg(2+)的逆转录酶活性，并能在人类CD4阳性细胞中诱导细胞病变效应。蛋白质印迹分析显示，SIVWCM的env产物与SIVAGM的env产物交叉反应比与HIV-1和SIVMAC的env产物交叉反应更强，并且用SIVAGM探针检测到了清晰的杂交带。为了将该病毒序列与其他灵长类慢病毒序列进行比较，对部分pol基因和长末端重复序列（LTR）进行了扩增和克隆。测序结果表明，SIVWCM分离株与SIVAGM分离株密切相关。这项研究表明，来自猕猴属的SIVAGM和来自白睑猴属的SIVWCM可能属于一个SIV组，该组在基因上与白冠白睑猴所属的白睑猴属的乌黑白睑猴（SIVSMM）的SIV不同。
