福尔马林固定石蜡包埋乳腺癌组织中EB病毒DNA的检测
Investigation of Epstein-Barr virus DNA in formalin-fixed and paraffin- embedded breast cancer tissues.
作者信息
Kalkan Ahmet, Ozdarendeli Aykut, Bulut Yasemin, Yekeler Hayrettin, Cobanoglu Bengu, Doymaz Mehmet Z
机构信息
Department of Infectious Diseases, Firat University Medical School, Elazig, Turkey.
出版信息
Med Princ Pract. 2005 Jul-Aug;14(4):268-71. doi: 10.1159/000085748.
OBJECTIVE
To investigate etiological role of Epstein-Barr virus (EBV) DNA in breast cancer.
MATERIALS AND METHODS
The presence of EBV DNA in 57 breast cancer tissues was investigated with a sensitive PCR assay. The breast cancer tissues were from invasive ductular (n=28), lobular (n=20) and other miscellaneous carcinomas (n=9). Tissues from normal breasts and patients with various benign breast diseases (n=55): fibrocystic disease (n=34), fibroadenoma (n=16), hyperplasia, and granulomatous mastitis (n=5), were used as control samples.
RESULTS
EBV DNA was detected in 13 (23%) cancerous tissues (7 ductular, 4 lobular, 2 other carcinoma) and 19 (35%) in the control tissues. The difference between EBV presence in malignant and benign tissues was not statistically significant (p>0.05).
CONCLUSION
The presence of EBV DNA was detected almost equally in both breast cancer and normal tissues, which indicates no etiological role for EBV in breast cancer. We suggest further etiological studies.
目的
探讨爱泼斯坦-巴尔病毒（EBV）DNA在乳腺癌中的病因学作用。
材料与方法
采用灵敏的聚合酶链反应（PCR）检测法，对57例乳腺癌组织中EBV DNA的存在情况进行研究。这些乳腺癌组织来自浸润性导管癌（n = 28）、小叶癌（n = 20）和其他杂类癌（n = 9）。取自正常乳房组织以及患有各种良性乳腺疾病的患者（n = 55）：纤维囊性疾病（n = 34）、纤维腺瘤（n = 16）、增生和肉芽肿性乳腺炎（n = 5）的组织用作对照样本。
结果
在13例（23%）癌组织（7例导管癌、4例小叶癌、2例其他癌）和19例（35%）对照组织中检测到EBV DNA。恶性组织和良性组织中EBV的存在差异无统计学意义（p>0.05）。
结论
在乳腺癌组织和正常组织中检测到EBV DNA的情况几乎相同，这表明EBV在乳腺癌中无病因学作用。我们建议进一步开展病因学研究。
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