Nagi Karim, Gupta Ishita, Jurdi Nawaf, Jabeen Ayesha, Yasmeen Amber, Batist Gerald, Vranic Semir, Al-Moustafa Ala-Eddin
College of Medicine, QU Health, Qatar University, PO Box 2713, Doha, Qatar.
Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, PO Box 2713, Doha, Qatar.
Cancer Cell Int. 2021 Jun 10;21(1):308. doi: 10.1186/s12935-021-02009-4.
High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein-Barr virus (EBV) to initiate and/or enhance the progression of several types of human carcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in human breast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women.
A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study.
We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile, we report that high-risk HPVs and EBV are co-present in 30/102 (29%) of the samples; more significantly, our results indicate that their co-presence is associated with tumor grade (p = 0.03).
Our data revealed that HPVs and EBV are present/co-present in human breast cancer where they may play an important role in its development and/or progression; thus, we believe that further investigations are essential to confirm and elucidate the presence/co-presence of these oncoviruses and the underlying mechanisms of their interaction in breast carcinogenesis.
高危型人乳头瘤病毒（HPV）存在，并可与爱泼斯坦-巴尔病毒（EBV）协同作用，引发和/或促进包括宫颈癌以及头颈癌在内的多种人类癌症的进展；与此同时，最近有人指出，这些致癌病毒可在人类乳腺癌中被检测到。因此，我们在此探究了黎巴嫩女性乳腺癌中高危型HPV和EBV的存在情况/共同存在情况。
对102例乳腺癌样本和14例正常乳腺组织进行HPV和EBV检测。本研究采用了聚合酶链反应（PCR）、免疫组织化学（IHC）分析以及组织微阵列（TMA）平台。
我们发现66/102（65%）的样本中存在HPV，而41/102（40%）的队列中存在EBV。此外，我们的数据显示，高危型HPV类型（52、35、58、45、16和51）在黎巴嫩女性乳腺癌中最为常见。同时，我们报告30/102（29%） 的样本中高危型HPV和EBV共同存在；更显著的是，我们的结果表明它们的共同存在与肿瘤分级相关（p = 0.03）。
我们的数据显示HPV和EBV存在于/共同存在于人类乳腺癌中，它们可能在乳腺癌的发生和/或进展中起重要作用；因此，我们认为进一步的研究对于确认和阐明这些致癌病毒的存在/共同存在情况以及它们在乳腺癌发生过程中相互作用的潜在机制至关重要。
