Lee Yong-Jin, Wagner Isaac D, Brice Mary E, Kevbrin Vadim V, Mills Gary L, Romanek Christopher S, Wiegel Juergen
Department of Microbiology, University of Georgia, Athens, GA 30602-2605, USA.
Extremophiles. 2005 Oct;9(5):375-83. doi: 10.1007/s00792-005-0453-4. Epub 2005 Jun 18.
A new group of anaerobic thermophilic bacteria was isolated from enrichment cultures obtained from deep sea sediments of Peru Margin collected during Leg 201 of the Ocean Drilling Program. A total of ten isolates were obtained from cores of 1-2 m below seafloor (mbsf) incubated at 60 degrees C: three isolates came from the sediment 426 m below sea level with a surface temperature of 9 degrees C (Site 1227), one from 252 m below sea level with a temperature of 12 degrees C (Site 1228), and six isolates under sulfate-reducing condition from the lower slope of the Peru Trench (Site 1230). Strain JW/IW-1228P from the Site 1228 and strain JW/YJL-1230-7/2 from the Site 1230 were chosen as representatives of the two identified clades. Based on the 16S rDNA sequence analysis, these isolates represent a novel group with Thermovenabulum and Caldanaerobacter as their closest relatives. The temperature range for growth was 52-76 degrees C with an optimum at around 68 degrees C for JW/IW-1228P and 43-76 degrees C with an optimum at around 64 degrees C for JW/YJL-1230-7/2. The pH(25C) range for growth was from 6.3 to 9.3 with an optimum at 7.5 for JW/IW-1228P and from 5 to 9.5 with an optimum at 7.9-8.4 for JW/YJL-1230-7/2. The salinity range for growth was from 0% to 6% (w/v) for JW/IW-1228P and from 0% to 4.5% (w/v) for JW/YJL-1230-7/2. The G+C [corrected] mol% of the genomic DNA was 46.3 +/- 0.7% (n = 4) for Thermosediminibacter oceani [corrected] JW/IW-1228PT [corrected] and 45.2 +/- 0.7 (n = 6) for Thermosediminibacter litoriperuensis [corrected] JW/YJL-1230-7/2T [corrected] DNA-DNA hybridization yielded 52% similarity between the two strains. According to 16S rRNA gene sequence analysis, the isolates are located within the family, Thermoanaerobacteriaceae. Based on their morphological and physiological properties and phylogenetic analysis, it is proposed that strain JW/IW-1228P(T) is placed into a novel taxa, Thermosediminibacter oceani, gen. nov., sp. nov. (DSM 16646(T)=ATCC BAA-1034(T)), and JW/YJL-1230-7/2(T) into Thermosediminibacter litoriperuensis sp. nov. (DSM 16647(T) =ATCC BAA-1035(T)).
从大洋钻探计划第201航次采集的秘鲁边缘深海沉积物富集培养物中分离出一组新的厌氧嗜热细菌。在60℃培养的海底以下1-2米岩芯中总共获得了10株分离株：3株分离株来自海平面以下426米、表面温度为9℃的沉积物（1227站点），1株来自海平面以下252米、温度为12℃的沉积物（1228站点），6株在硫酸盐还原条件下从秘鲁海沟下坡处（1230站点）分离得到。来自1228站点的JW/IW-1228P菌株和来自1230站点的JW/YJL-1230-7/2菌株被选为两个已鉴定进化枝的代表。基于16S rDNA序列分析，这些分离株代表了一个新类群，与嗜热嗜温菌属和热厌氧杆菌属亲缘关系最近。JW/IW-1228P的生长温度范围为52-76℃，最适温度约为68℃；JW/YJL-1230-7/2的生长温度范围为43-76℃，最适温度约为64℃。JW/IW-1228P在pH(25℃) 6.3至9.3范围内生长，最适pH为7.5；JW/YJL-1230-7/2在pH 5至9.5范围内生长，最适pH为7.9-8.4。JW/IW-1228P的生长盐度范围为0%至6%（w/v），JW/YJL-1230-7/2的生长盐度范围为0%至4.5%（w/v）。海洋嗜热沉积物杆菌JW/IW-1228PT基因组DNA的G+C [校正] mol%为46.3±0.7%（n = 4），秘鲁海岸嗜热沉积物杆菌JW/YJL-1230-7/2T DNA的G+C [校正] mol%为45.2±0.7（n = 6）。两菌株间的DNA-DNA杂交相似性为52%。根据16S rRNA基因序列分析，这些分离株属于热厌氧杆菌科。基于它们的形态和生理特性以及系统发育分析，建议将JW/IW-1228P(T)菌株归入一个新分类单元，海洋嗜热沉积物杆菌属，新属，新种（DSM 16646(T)=ATCC BAA-1034(T)），将JW/YJL-1230-7/2(T)菌株归入秘鲁海岸嗜热沉积物杆菌新种（DSM 16647(T) =ATCC BAA-1035(T)）。
