Rauchensteiner Florian, Matsumura Yuki, Yamamoto Yutaka, Yamaji Seiichi, Tani Tadato
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
J Pharm Biomed Anal. 2005 Jul 15;38(4):594-600. doi: 10.1016/j.jpba.2005.01.038. Epub 2005 Mar 16.
A simple capillary-zone electrophoresis (CZE) method for the analysis of plant specimens, Glycyrrhiza glabra L., G. uralensisFisch. and G. inflata Bat. (Leguminosae) as well as commercial licorices from Europe and China was developed. Contents of glycyrrhizin (GL), glycyrrhetic acid (GA), glabridin (GLAB), liquiritin (LQ) and licochalcone A (LC(A)) in ethanolic extracts were investigated. Optimum separation was achieved with sodium tetraborate buffer (pH 9.22; 70 mM); voltage, 25 kV. Recovery rate for GL was found to be 101.90+/-2.54%. Adequate correlation was observed between GL contents measured by CZE and HPLC (r=0.977). Advantages over conventional HPLC analysis of Glycyrrhiza species are short analysis time (<15 min), simple running buffer preparation and the none-use of organic solvents. Using the present CZE method, it was demonstrated that (1) G. glabra was distinguished from G. uralensis especially by phenolic compounds GLAB (G. glabra: 0.19+/-0.11%; n=53) and LQ (G. uralensis, 1.34+/-0.34%, n=10); (2) on average, GL contents were higher in Chinese commercial licorices; (3) relatively high LC(A) contents were especially detected in a Chinese commercial licorice (origin estimated as G. inflata); (4) Glycyrrhiza species were also distinguished by applying PCA on the basis of CZE peak area data of GL, GLAB, GA, LQ and LC(A); and (5) liquiritin apioside was found in all samples.
开发了一种简单的毛细管区带电泳（CZE）方法，用于分析植物标本光果甘草、乌拉尔甘草和胀果甘草（豆科）以及来自欧洲和中国的市售甘草。研究了乙醇提取物中甘草酸（GL）、甘草次酸（GA）、光甘草定（GLAB）、甘草苷（LQ）和甘草查尔酮A（LC(A)）的含量。使用硼酸钠缓冲液（pH 9.22；70 mM）实现了最佳分离；电压为25 kV。发现GL的回收率为101.90±2.54%。通过CZE和HPLC测定的GL含量之间观察到充分的相关性（r=0.977）。与传统的甘草属物种HPLC分析相比，优势在于分析时间短（<15分钟）、运行缓冲液制备简单且不使用有机溶剂。使用目前的CZE方法证明：（1）光果甘草与乌拉尔甘草的区别尤其在于酚类化合物GLAB（光果甘草：0.19±0.11%；n=53）和LQ（乌拉尔甘草，1.34±0.34%，n=10）；（2）中国市售甘草中GL含量平均较高；（3）在中国一种市售甘草（估计来源为胀果甘草）中特别检测到相对较高的LC(A)含量；（4）基于GL、GLAB、GA、LQ和LC(A)的CZE峰面积数据应用主成分分析（PCA）也能区分甘草属物种；（5）在所有样品中均发现了甘草苷芹菜糖苷。
