Reichert T, Störkel S, Becker K, Fisher L W
Department of Pathology, University of Mainz, FRG.
Calcif Tissue Int. 1992 May;50(5):468-72. doi: 10.1007/BF00296779.
We investigated immunohistologically 160 teeth and dental germs in various stages of tooth development taken from human individuals (13th week of pregnancy to the 24th year of life) to study the osteonectin expression in dental hard tissue. In the course of dentinogenesis, the predentin, the odontoblasts, and their cell processes show a positive osteonectin staining reaction. During cementogenesis, osteonectin is synthesized by cement-producing fibroblasts, cementoblasts, and cementocytes. The expression of osteonectin during dentinogenesis and cementogenesis is closely related to the development of the respective calcified tissue. All cells of the inner and outer enamel epithelium, the cells of the stratum reticulare and stratum intermedium, the ameloblasts, and the enamel substance are osteonectin negative, just as dentin and cement are. The results of this study indicate one important physiological role of osteonectin as a protein associated with the formation of collagen containing mineralizing tissues like human bone, as well as human dentin and cement.
我们对取自人类个体（孕期第13周至24岁）不同牙齿发育阶段的160颗牙齿及牙胚进行了免疫组织学研究，以探讨骨连接蛋白在牙齿硬组织中的表达情况。在牙本质形成过程中，前期牙本质、成牙本质细胞及其细胞突起呈现骨连接蛋白阳性染色反应。在牙骨质形成过程中，骨连接蛋白由产生牙骨质的成纤维细胞、成牙骨质细胞和牙骨质细胞合成。牙本质形成和牙骨质形成过程中骨连接蛋白的表达与各自钙化组织的发育密切相关。内釉上皮和外釉上皮的所有细胞、星网状层和中间层的细胞、成釉细胞以及釉质均为骨连接蛋白阴性，牙本质和牙骨质也是如此。本研究结果表明，骨连接蛋白作为一种与含胶原矿化组织（如人体骨骼、牙本质和牙骨质）形成相关的蛋白质，具有重要的生理作用。
