Zhu Linyong, Zhu Ming-Qiang, Hurst James K, Li Alexander D Q
Department of Chemistry and Center for Materials Research, Washington State University, Pullman, Washington 99164.
J Am Chem Soc. 2005 Jun 29;127(25):8968-70. doi: 10.1021/ja0423421.
Spiropyran dyes were attached to fluorescent core-shell CdSe/ZnS nanocrystals via thiol-containing linkers. Photoisomerization of the dye to its merocyanine form by UV irradiation caused a dramatic loss in the intrinsic nanoparticle fluorescence, which was regained upon reversing the isomerization with visible light. The fluorescence quenching efficiency increased with increasing spectral overlap of fluorescence emission and merocyanine adsorption bands, consistent with FRET as the quenching mechanism. Typically, complete quenching required at least 80 bound dye molecules per particle.
通过含硫醇的连接体将螺吡喃染料连接到荧光核壳型CdSe/ZnS纳米晶体上。通过紫外线照射使染料光异构化为部花青形式会导致纳米颗粒固有荧光急剧损失，而用可见光使异构化逆转时荧光会恢复。荧光猝灭效率随着荧光发射光谱与部花青吸附带光谱重叠的增加而提高，这与作为猝灭机制的荧光共振能量转移一致。通常，每个颗粒完全猝灭至少需要80个结合的染料分子。
