van der Kuyl Antoinette C, Polstra Abeltje M, van den Burg Remco, Jan Weverling Gerrit, Goudsmit Jaap, Cornelissen Marion
Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
J Med Virol. 2005 Aug;76(4):541-6. doi: 10.1002/jmv.20396.
To establish the effect of the presence in blood cells of cytomegalovirus (CMV) and human herpesvirus 8 (HHV8) DNA, two herpesviruses that are activated frequently in AIDS patients, were selected from the Amsterdam Cohort Studies on HIV/AIDS 181 PBMC samples from patients with and without Kaposi's sarcoma (KS), and with and without CMV-related disease. The viral loads of both HHV8 and CMV were determined by real-time PCR at the time of diagnosis of AIDS. There was no significant difference in prevalence and load for CMV between the KS and non-KS patients. The variable related most strongly to KS was the presence of HHV8 DNA in PBMCs, whilst CMV DNA was related to the development of CMV disease and shortened survival. The frequency of detection of HHV8 increased when the patient presented with more severe KS symptoms at diagnosis, but detection of HHV8 DNA did not influence survival. Therefore, HHV8 and CMV DNA measured in the blood of AIDS patients, are each related mainly to the associated disease, and have no additional predictive value in these patients.
为确定血细胞中巨细胞病毒（CMV）和人类疱疹病毒8型（HHV8）DNA的存在所产生的影响，从阿姆斯特丹HIV/AIDS队列研究中选取了181份来自有或没有卡波西肉瘤（KS）以及有或没有CMV相关疾病的患者的外周血单个核细胞（PBMC）样本，这两种疱疹病毒在艾滋病患者中经常被激活。在艾滋病诊断时通过实时聚合酶链反应（PCR）测定HHV8和CMV的病毒载量。KS患者和非KS患者之间CMV的患病率和载量没有显著差异。与KS最密切相关的变量是PBMC中HHV8 DNA的存在，而CMV DNA与CMV疾病的发展和生存期缩短有关。当患者在诊断时出现更严重的KS症状时，HHV8的检测频率增加，但HHV8 DNA的检测并不影响生存期。因此，在艾滋病患者血液中检测到的HHV8和CMV DNA，各自主要与相关疾病有关，在这些患者中没有额外的预测价值。
