Devor Marshall, Gilad Amit, Arbilly Michal, Yakir Benjamin, Raber Pnina, Pisanté Anne, Darvasi Ariel
Department of Cell and Animal Biology, and Center for Research on Pain, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel Department of Statistics, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Pain. 2005 Aug;116(3):289-293. doi: 10.1016/j.pain.2005.04.023.
We have produced a backcross (BC) population of 267 mice from the parental strains C3H/HeN and C58/J. The mice were phenotyped for neuropathic pain using the neuroma model. Subsequently all BC mice were genotyped in a region of chromosome 15 that has been previously suggested to contain a quantitative trait locus (QTL) for this trait. We have confirmed the linkage of the QTL, named pain1, to the central region of chromosome 15. Our finding provides the necessary robustness to justify efforts towards identification of the underlying gene.
我们利用亲本品系C3H/HeN和C58/J培育出了一个由267只小鼠组成的回交（BC）群体。使用神经瘤模型对这些小鼠进行神经性疼痛的表型分析。随后，对所有BC小鼠在15号染色体的一个区域进行基因分型，该区域先前被认为含有控制此性状的数量性状基因座（QTL）。我们已经证实了名为pain1的QTL与15号染色体中央区域的连锁关系。我们的发现提供了必要的可信度，为鉴定潜在基因的工作提供了依据。
