Chakrabarti S, Poddar M K
Department of Biochemistry, University of Calcutta, India.
Methods Find Exp Clin Pharmacol. 1992 Mar;14(2):115-23.
Single electroacupuncture (EA) exposure (10 Hz, 1 volt, 15 min) to long-term (15 consecutive days) diazepam (5 mg/kg/day, i.p.) treated adult male albino rats (120-140 g) potentiated the long-term diazepam-induced increase of GABAergic activity in thalamus (Th) but the long-term diazepam-induced inhibition of GABAergic activity in cerebral cortex (CC) and spinal cord (SC) was disinhibited under similar condition of treatment. These changes in brain regional GABAergic activity may be correlated with the inhibition of single EA-induced analgesia (EAA) under similar conditions of long-term diazepam treatment with single EA exposure. Single EA exposure alone, however, inhibited GABAergic activity in Th and pons-medulla (PM) with the development of EAA. Multiple EA (10 Hz, 1 volt, 10 min/day) exposure along with long-term diazepam for 15 consecutive days inhibited GABAergic activity in CC and SC which is similar to the effect observed with either multiple EA exposure or long-term diazepam alone. But in Th the multiple EA or long-term diazepam-induced increase in GABAergic activity was significantly potentiated with the co-treatment of multiple EA and long-term diazepam for 15 consecutive days. Although GABAergic activity in PM under multiple EA or long-term diazepam treatment alone was not affected their (EA and diazepam) co-exposures under long-term conditions significantly enhanced GABAergic activity in PM. These region specific characteristic changes in central GABAergic activity under long-term treatment of diazepam along with EA may thus explain the potentiation of multiple EA or long-term diazepam-induced analgesia (tail flick latency).
对长期（连续15天）接受地西泮（5毫克/千克/天，腹腔注射）治疗的成年雄性白化大鼠（120 - 140克）进行单次电针（EA）刺激（10赫兹，1伏特，15分钟），可增强长期地西泮诱导的丘脑（Th）中γ-氨基丁酸能（GABAergic）活性的增加，但在类似治疗条件下，长期地西泮诱导的大脑皮质（CC）和脊髓（SC）中GABAergic活性的抑制被解除抑制。在长期地西泮与单次EA暴露的类似条件下，脑区GABAergic活性的这些变化可能与单次EA诱导的镇痛作用（EAA）的抑制有关。然而，单独的单次EA暴露会随着EAA的产生抑制Th和脑桥-延髓（PM）中的GABAergic活性。连续15天多次EA（10赫兹，1伏特，每天10分钟）暴露与长期地西泮联合使用，可抑制CC和SC中的GABAergic活性，这与单独多次EA暴露或长期地西泮所观察到的效果相似。但在Th中，连续15天多次EA与长期地西泮联合治疗可显著增强多次EA或长期地西泮诱导的GABAergic活性增加。尽管单独的多次EA或长期地西泮治疗下PM中的GABAergic活性未受影响，但在长期条件下它们（EA和地西泮）的共同暴露显著增强了PM中的GABAergic活性。因此，在长期地西泮与EA联合治疗下，中枢GABAergic活性的这些区域特异性特征变化可能解释了多次EA或长期地西泮诱导的镇痛作用（甩尾潜伏期）的增强。
